ArticlePloS one2018
Demonstration of translation elongation factor 3 activity from a non-fungal species, Phytophthora infestans.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- The geneFrontiers in microbiology · 2024Article
- Comparative Proteomic Analysis ofJournal of fungi (Basel, Switzerland) · 2022Article
- Frameshifting at collided ribosomes is modulated by elongation factor eEF3 and by integrated stress response regulators Gcn1 and Gcn20.RNA (New York, N.Y.) · 2022Article
- Mutational analysis reveals potential phosphorylation sites in eukaryotic elongation factor 1A that are important for its activity.FEBS letters · 2021Article
- Assessment of the mechanism of drug resistance in Trichophyton mentagrophytes in response to various substances.BMC genomics · 2021Article
- Yeast translation elongation factor eEF3 promotes late stages of tRNA translocation.The EMBO journal · 2021Article
- Eukaryotic Elongation Factor 3 ProtectsGenes · 2020Article
- Diversity of tRNA Clusters in the Chloroviruses.Viruses · 2020Article
- A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling.Nucleic acids research · 2019Article
- Evaluation and Validation of Reference Genes for Quantitative Real-Time PCR in Helopeltis theivora Waterhouse (Hemiptera: Miridae).Scientific reports · 2019Article
- Ribosome profiling analysis of eEF3-depleted Saccharomyces cerevisiae.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In most eukaryotic organisms, translation elongation requires two highly conserved elongation factors eEF1A and eEF2. Fungal systems are unique in requiring a third factor, the eukaryotic Elongation Factor 3 (eEF3). For decades, eEF3, a ribosome-dependent ATPase, was considered "fungal-specific", however, recent bioinformatics analysis indicates it may be more widely distributed among other unicellular eukaryotes. In order to determine whether divergent eEF3-like proteins from other eukaryotic organisms can provide the essential functions of eEF3 in budding yeast, the eEF3-like proteins from Schizosaccharomyes pombe and an oomycete, Phytophthora infestans, were cloned and expressed in Saccharomyces cerevisiae. Plasmid shuffling experiments showed that both S. pombe and P. infestans eEF3 can support the growth of S. cerevisiae in the absence of endogenous budding yeast eEF3. Consistent with its ability to provide the essential functions of eEF3, P. infestans eEF3 possessed ribosome-dependent ATPase activity. Yeast cells expressing P. infestans eEF3 displayed reduced protein synthesis due to defects in translation elongation/termination. Identification of eEF3 in divergent species will advance understanding of its function and the ribosome specific determinants that lead to its requirement as well as contribute to the identification of functional domains of eEF3 for potential drug discovery.
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