ArticleNature protocols2018
Use of a three-layer gradient system of cells for rat testicular organoid generation.
Article in Nature protocols, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 54 citations in OpenAlex.
- Associations of Bovine Fetal Adipose Tissue-Derived Mesenchymal Stem/Stromal Cells and Germ Cell Marker Expression in Testicular Organoids.Animals : an open access journal from MDPI · 2026Article
- Ex Vivo Spatiotemporal Characterization of Spermatogenesis in Mouse Testicular Organoids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Differential retinoic acid responses across testicular developmentbioRxiv : the preprint server for biology · 2025Article
- In vitro models of the male reproductive system: applications for developmental and reproductive toxicology.Toxicological sciences : an official journal of the Society of Toxicology · 2025Review
- Modeling antithymocyte globulin-induced microvasculopathy using human iPSC-derived vascularized liver organoids.Cell reports. Medicine · 2025Article
- Self-organization of sinusoidal vessels in pluripotent stem cell-derived human liver bud organoids.Nature biomedical engineering · 2025Article
- Identification and isolation of human testicular peritubular myoid cells and Leydig cells by a combination of ITGA9 and NGFR.Reproductive biology and endocrinology : RB&E · 2025Article
- Mechanical signal-mediated mitochondria-endoplasmic reticulum contacts modulate Leydig cell testosterone biosynthesis during testis development.Molecular human reproduction · 2025Article
- Comparative Single-Cell Transcriptomics of Human Neuroblastoma and Preclinical Models Reveals Conservation of an Adrenergic Cell State.Cancer research · 2025Article
- A comparative analysis of vitrification and two slow freezing methods for gonocyte-containing neonatal calf testicular tissue and subsequent in vitro culture.In vitro models · 2025Article
- Silk-Ovarioids: establishment and characterization of a human ovarian primary cell 3D-model system.Human reproduction open · 2025Article
- Spatially defined microenvironment for engineering organoids.Biophysics reviews · 2024Review
- Article
- Review
- Male Infertility: New Developments, Current Challenges, and Future Directions.The world journal of men's health · 2024Review
- Frequency, morbidity and equity - the case for increased research on male fertility.Nature reviews. Urology · 2024Review
- Prior exposure to alkylating agents negatively impacts testicular organoid formation in cells obtained from childhood cancer patients.Human reproduction open · 2024Article
- In vitro spermatogenesis in artificial testis: current knowledge and clinical implications for male infertility.Cell and tissue research · 2023Review
- Review
- Biomaterial strategies for the application of reproductive tissue engineering.Bioactive materials · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have recently developed a 3D culture system that allows the reorganization of rat primary testicular cells into organoids with a functional blood-testis barrier, as well as the establishment and maintenance of germ cells. The innovative aspect of our model, the three-layer gradient system (3-LGS), comprises cells combined with Matrigel placed between two layers of Matrigel without cells, which creates a gradient of cells and allows the reorganization of testicular cells into organized structures after 5-7 d in culture. This reorganization is not observed when testicular cells are suspended in only one layer of Matrigel, the methodology used in the majority of the protocols for generating organoids. The model can be applied to follow and quantify cell migration during testicular organoid formation, and to explore the role of growth factors and the toxic effects of drugs and environmental contaminants on germ cell maintenance and blood-testis barrier integrity. The 3-LGS is a robust and reproducible method that requires a small volume of Matrigel and a low number of cells (16 μl and 132,000 cells, respectively), enabling and facilitating high-throughput analysis of germ-to-somatic cell associations in vitro.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.