Evidence map›Paper›PMID 29300364›Full record

ArticleInternational journal of molecular sciences2018

ФC31 Integrase-Mediated Isolation and Characterization of Novel Safe Harbors for Transgene Expression in the Pig Genome.

Yanzhen Bi, Zaidong Hua, Hongyan Ren, Liping Zhang, Hongwei Xiao, Ximei Liu, Wenjun Hua, Shuqi Mei, Adrian Molenaar, Götz Laible and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Yanzhen BiHubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Hubei Institute of Animal Science and Veterinary Medicine, Hubei Academy of AgroSciences, Wuhan 430064, China. sukerbyz@126.com.
Zaidong HuaHubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Hubei Institute of Animal Science and Veterinary Medicine, Hubei Academy of AgroSciences, Wuhan 430064, China. zaidonghua@163.com.
Hongyan RenHubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Hubei Institute of Animal Science and Veterinary Medicine, Hubei Academy of AgroSciences, Wuhan 430064, China. renhongyan507@163.com.
Liping ZhangHubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Hubei Institute of Animal Science and Veterinary Medicine, Hubei Academy of AgroSciences, Wuhan 430064, China. chzlp1982@163.com.
Hongwei XiaoHubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Hubei Institute of Animal Science and Veterinary Medicine, Hubei Academy of AgroSciences, Wuhan 430064, China. xiaohongwei2003@163.com.
Ximei LiuHubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Hubei Institute of Animal Science and Veterinary Medicine, Hubei Academy of AgroSciences, Wuhan 430064, China. anbit20@hotmail.com.
Wenjun HuaHubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Hubei Institute of Animal Science and Veterinary Medicine, Hubei Academy of AgroSciences, Wuhan 430064, China. huawenjun08@aliyun.com.
Shuqi MeiHubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Hubei Institute of Animal Science and Veterinary Medicine, Hubei Academy of AgroSciences, Wuhan 430064, China. msqlfe@163.com.
Adrian MolenaarAgResearch Ltd., Grasslands Research Centre, Private Bag 11008, Palmerston North 4442, New Zealand. adrian.molenaar@agresearch.co.nz.
Götz LaibleAgResearch Ltd., Ruakura Research Centre, Private Bag 3123, Hamilton 3214, New Zealand. goetz.laible@agresearch.co.nz.
Xinmin ZhengHubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Hubei Institute of Animal Science and Veterinary Medicine, Hubei Academy of AgroSciences, Wuhan 430064, China. novorigin@126.com.
Hubei Academy of Agricultural Sciences · CNAgResearch · NZ

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Programmable nucleases have allowed the rapid development of gene editing and transgenics, but the technology still suffers from the lack of predefined genetic loci for reliable transgene expression and maintenance. To address this issue, we used ФC31 integrase to navigate the porcine genome and identify the pseudo attP sites suitable as safe harbors for sustained transgene expression. The combined ФC31 integrase mRNA and an enhanced green fluorescence protein (EGFP) reporter donor were microinjected into one-cell zygotes for transgene integration. Among the resulting seven EGFP-positive piglets, two had transgene integrations at pseudo attP sites, located in an intergenic region of chromosome 1 (chr1-attP) and the 6th intron of the

Indexed as

Gene ExpressionGenomeTransgenesAnimalsAnimals, Genetically ModifiedBiocatalysisEmbryo, MammalianIntegrasesMicroinjectionsRecombination, GeneticSiphoviridaeSus scrofaTissue DonorsZygoteIntegrasespigpseudo attP sitesafe harbortransgeneФC31 integrase

Identifiers

PMID29300364
PMCPMC5796098
OpenAlexW2782251183

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.