ArticleLab on a chip2018
Linking invasive motility to protein expression in single tumor cells.
Article in Lab on a chip, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- DropBlot: single-cell western blotting of chemically fixed cancer cells.Nature communications · 2024Article
- Matrix obstructions cause multiscale disruption in collective epithelial migration by suppressing leader cell function.Molecular biology of the cell · 2023Article
- Microphysiological systems to study tumor-stroma interactions in brain cancer.Brain research bulletin · 2021Review
- Phenotypic Heterogeneity and Metastasis of Breast Cancer Cells.Cancer research · 2021Article
- A microfluidic cell-migration assay for the prediction of progression-free survival and recurrence time of patients with glioblastoma.Nature biomedical engineering · 2021Article
- Review
- 3D projection electrophoresis for single-cell immunoblotting.Nature communications · 2020Article
- Two high-yield complementary methods to sort cell populations by their 2D or 3D migration speed.Molecular biology of the cell · 2020Article
- A mode of cell adhesion and migration facilitated by CD44-dependent microtentacles.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Clonal ZEB1-Driven Mesenchymal Transition Promotes Targetable Oncologic Antiangiogenic Therapy Resistance.Cancer research · 2020Article
- Suppression of LIM Kinase 1 and LIM Kinase 2 Limits Glioblastoma Invasion.Cancer research · 2020Article
- Transcriptomic analysis reveals that BMP4 sensitizes glioblastoma tumor-initiating cells to mechanical cues.Matrix biology : journal of the International Society for Matrix Biology · 2020Article
- Controlling Dispersion during Single-Cell Polyacrylamide-Gel Electrophoresis in Open Microfluidic Devices.Analytical chemistry · 2018Article
- Microfluidic Analyzer Enabling Quantitative Measurements of Specific Intracellular Proteins at the Single-Cell Level.Micromachines · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
Abstract
The invasion of malignant cells into tissue is a critical step in the progression of cancer. While it is increasingly appreciated that cells within a tumor differ in their invasive potential, it remains nearly unknown how these differences relate to cell-to-cell variations in protein expression. Here, we introduce a microfluidic platform that integrates measurements of invasive motility and protein expression for single cells, which we use to scrutinize human glioblastoma tumor-initiating cells (TICs). Our live-cell imaging microdevice is comprised of polyacrylamide microchannels that exhibit tissue-like stiffness and present chemokine gradients along each channel. Due to intrinsic differences in motility, cell subpopulations separate along the channel axis. The separated cells are then lysed in situ and each single-cell lysate is subjected to western blotting in the surrounding polyacrylamide matrix. We observe correlations between motility and Nestin and EphA2 expression. We identify protein-protein correlations within single TICs, which would be obscured with population-based assays. The integration of motility traits with single-cell protein analysis - on the same cell - offers a new means to identify druggable targets of invasive capacity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.