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Cooperating H3N2 Influenza Virus Variants Are Not Detectable in Primary Clinical Samples.
Article in mSphere. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Receptor Binding Properties of Neuraminidase for influenza A virus: An Overview of Recent Research Advances.Virulence · 2023Review
- Open questions in the social lives of viruses.Journal of evolutionary biology · 2023Review
- Antivirals Targeting the Neuraminidase.Cold Spring Harbor perspectives in medicine · 2022Review
- Principles of dengue virus evolvability derived from genotype-fitness maps in human and mosquito cells.eLife · 2021Article
- The Ecology and Evolution of Influenza Viruses.Cold Spring Harbor perspectives in medicine · 2020Review
- Antibody Neutralization of an Influenza Virus that Uses Neuraminidase for Receptor Binding.Viruses · 2020Article
- Passage of influenza A/H3N2 viruses in human airway cells removes artefactual variants associated with neuraminidase-mediated binding.The Journal of general virology · 2020Article
- Protein-protein interactions of human viruses.Seminars in cell & developmental biology · 2020Review
- Reconciling disparate estimates of viral genetic diversity during human influenza infections.Nature genetics · 2019Article
- Uncovering Virus-Virus Interactions by Unifying Approaches and Harnessing High-Throughput Tools.mSystems · 2019Article
- The secret social lives of viruses.Nature · 2019Article
- Influenza Neuraminidase: Underrated Role in Receptor Binding.Trends in microbiology · 2019Review
- Evolutionary Virology at 40.Genetics · 2018Review
- Mechanisms and Concepts in RNA Virus Population Dynamics and Evolution.Annual review of virology · 2018Review
- Article
- Beneficial coinfection can promote within-host viral diversity.Virus evolution · 2018Article
- An optimized methodology for whole genome sequencing of RNA respiratory viruses from nasopharyngeal aspirates.PloS one · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The high mutation rates of RNA viruses lead to rapid genetic diversification, which can enable cooperative interactions between variants in a viral population. We previously described two distinct variants of H3N2 influenza virus that cooperate in cell culture. These variants differ by a single mutation, D151G, in the neuraminidase protein. The D151G mutation reaches a stable frequency of about 50% when virus is passaged in cell culture. However, it is unclear whether selection for the cooperative benefits of D151G is a cell culture phenomenon or whether the mutation is also sometimes present at appreciable frequency in virus populations sampled directly from infected humans. Prior work has not detected D151G in unpassaged clinical samples, but those studies have used methods like Sanger sequencing and pyrosequencing, which are relatively insensitive to low-frequency variation. We identified nine samples of human H3N2 influenza virus collected between 2013 and 2015 in which Sanger sequencing had detected a high frequency of the D151G mutation following one to three passages in cell culture. We deep sequenced the unpassaged clinical samples to identify low-frequency viral variants. The frequency of D151G did not exceed the frequency of library preparation and sequencing errors in any of the sequenced samples. We conclude that passage in cell culture is primarily responsible for the frequent observations of D151G in recent H3N2 influenza virus strains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.