ArticleApplications in plant sciences2017
Using herbarium-derived DNAs to assemble a large-scale DNA barcode library for the vascular plants of Canada.
Article in Applications in plant sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed.
- From Permits to Samples: Addressing Key Challenges for High-Quality Reference Genome Generation in Europe.Molecular ecology resources · 2026Review
- A revision ofIMA fungus · 2026Article
- A DNA Barcode Reference Library of Native Seed Plants in Chinese Dryland Regions.Scientific data · 2025Article
- A composite universal DNA signature for the tree of life.Nature ecology & evolution · 2025Article
- Comparative Analysis of Pasture Composition: DNA Metabarcoding Versus Quadrat-Based Botanical Surveys in Experimental Grassland Plots.Ecology and evolution · 2025Article
- How new plant species have been discovered in China: collection gaps and preferences over the past century.Frontiers in plant science · 2025Article
- PhytoKeys · 2025Article
- Confusing boundaries of the Labrador tea species: dispersal history explains the lack of clear species structure.Annals of botany · 2024Article
- Plants, pollinators and their interactions under global ecological change: The role of pollen DNA metabarcoding.Molecular ecology · 2023Article
- Phylogenetic relationships inPhytoKeys · 2022Article
- A phylogenetic analysis ofPeerJ · 2022Article
- Evolutionary history and patterns of geographical variation, fertility, and hybridization inFrontiers in plant science · 2022Article
- Molecular and morphological survey of Lamiaceae species in converted landscapes in Sumatra.PloS one · 2022Article
- Variability in ITS1 and ITS2 sequences of historic herbaria and extant (fresh) Phalaris species (Poaceae).BMC plant biology · 2021Article
- DNA-Based Herbal Teas' Authentication: An ITS2 andPlants (Basel, Switzerland) · 2021Article
- DNA barcoding of native Caucasus herbal plants: potentials and limitations in complex groups and implications for phylogeographic patterns.Biodiversity data journal · 2021Article
- The Effects of Herbarium Specimen Characteristics on Short-Read NGS Sequencing Success in Nearly 8000 Specimens: Old, Degraded Samples Have Lower DNA Yields but Consistent Sequencing Success.Frontiers in plant science · 2021Article
- The Treasure Vault Can be Opened: Large-Scale Genome Skimming Works Well Using Herbarium and Silica Gel Dried Material.Plants (Basel, Switzerland) · 2020Article
- Editorial: Integrative and Translational Uses of Herbarium Collections Across Time, Space, and Species.Frontiers in plant science · 2020Article
- Factors Affecting Targeted Sequencing of 353 Nuclear Genes From Herbarium Specimens Spanning the Diversity of Angiosperms.Frontiers in plant science · 2019Article
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26 authors.
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Abstract
premise of the studyConstructing complete, accurate plant DNA barcode reference libraries can be logistically challenging for large-scale floras. Here we demonstrate the promise and challenges of using herbarium collections for building a DNA barcode reference library for the vascular plant flora of Canada.
methodsOur study examined 20,816 specimens representing 5076 of 5190 vascular plant species in Canada (98%). For 98% of the specimens, at least one of the DNA barcode regions was recovered from the plastid loci
resultsSpecimen age and method of preservation had significant effects on sequence recovery for all markers, but influenced some families more (e.g., Boraginaceae) than others (e.g., Asteraceae). DISCUSSION: Our DNA barcode library represents an unparalleled resource for metagenomic and ecological genetic research working on temperate and arctic biomes. An observed decline in sequence recovery with specimen age may be associated with poor primer matches, intragenomic variation (for ITS2), or inhibitory secondary compounds in some taxa.
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