Evidence map›Paper›PMID 29298898›Full record

ArticleThe Journal of biological chemistry2018

A triad of residues is functionally transferrable between 5-HT

Richard Mosesso, Dennis A Dougherty

Open access · hybridAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Richard MosessoDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125.
Dennis A DoughertyDivision of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125. Electronic address: dadoc@caltech.edu.
California Institute of Technology · US

Funding

PREDOCTORAL TRAINING IN BIOLOGY AND CHEMISTRYT32GM007616 · NIGMS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI STERNBERG, PAUL WARREN · 1985 to 2019
$22.9M
PROBING RECEPTOR STRUCTURES WITH UNNATURAL AMINO ACIDSR01NS034407 · NINDS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI DOUGHERTY, DENNIS A · 1995 to 2016
$3.1M
Probing Receptor Structures with Unnatural Amino AcidsR37NS034407 · NINDS · CALIFORNIA INSTITUTE OF TECHNOLOGY · PI DOUGHERTY, DENNIS A · 2004 to 2010
$2.7M
NIGMS NIH HHS T32 GM007616NINDS NIH HHS R01 NS034407NINDS NIH HHS R37 NS034407
6 · The paper itself

Abstract

Cys-loop receptors are pentameric ligand-gated ion channels that facilitate communication within the nervous system. Upon neurotransmitter binding, these receptors undergo an allosteric activation mechanism connecting the binding event to the membrane-spanning channel pore, which expands to conduct ions. Some of the earliest steps in this activation mechanism are carried out by residues proximal to the binding site, the relative positioning of which may reflect functional differences among members of the Cys-loop family of receptors. Herein, we investigated key side-chain interactions near the binding site via mutagenesis and two-electrode voltage-clamp electrophysiology in serotonin-gated 5-HT

Indexed as

Models, MolecularAcetylcholineAmino Acid MotifsAmino Acid SequenceAmino Acid SubstitutionAnimalsBinding, CompetitiveBinding SitesCholinergic AgonistsHumansKineticsLigandsMiceMutagenesis, Site-DirectedMutationNicotineAcetylcholineCholinergic AgonistsHTR3A protein, humanLigandsNicotineNicotinic AntagonistsReceptors, NicotinicReceptors, Serotonin, 5-HT3Recombinant ProteinsCys-loop receptorelectrophysiologygatingligand-binding proteinmutagenesisnicotinic acetylcholine receptors (nAChR)serotoninstructure-function

Identifiers

PMID29298898
PMCPMC5827437
OpenAlexW2781727979

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.