Evidence map›Paper›PMID 29298406›Full record

ArticleTissue engineering. Part C, Methods2018

A Miniature Swine Model for Stem Cell-Based De Novo Regeneration of Dental Pulp and Dentin-Like Tissue.

Xiaofei Zhu, Jie Liu, Zongdong Yu, Chao-An Chen, Hacer Aksel, Adham A Azim, George T-J Huang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Tissue engineering. Part C, Methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed
10.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed, 95 citations in OpenAlex.

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  12. Research progress of dental pulp regeneration treatment.Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 4 countries.

Xiaofei Zhu1 Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center , Memphis, Tennessee.
Jie Liu1 Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center , Memphis, Tennessee.
Zongdong Yu1 Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center , Memphis, Tennessee.
Chao-An Chen1 Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center , Memphis, Tennessee.
Hacer Aksel1 Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center , Memphis, Tennessee.
Adham A Azim1 Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center , Memphis, Tennessee.
George T-J Huang1 Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center , Memphis, Tennessee.
University of Tennessee Health Science Center · USChi Mei Medical Center · TWHacettepe University · TRPeking University · CN

Funding

Stem Cell Based Therapy for Regenerative Endodontics R01DE019156 · NIDCR · UNIVERSITY OF TENNESSEE HEALTH SCI CTR · PI HUANG, GEORGE T.J · 2008 to 2013
$1.8M
NIDCR NIH HHS R01 DE019156
6 · The paper itself

Abstract

The goal of this study was to establish mini-swine as a large animal model for stem cell-based pulp regeneration studies. Swine dental pulp stem cells (sDPSCs) were isolated from mini-swine and characterized in vitro. For in vivo studies, we first employed both ectopic and semi-orthotopic study models using severe combined immunodeficiency mice. One is hydroxyapatite-tricalcium phosphate (HA/TCP) model for pulp-dentin complex formation, and the other is tooth fragment model for complete pulp regeneration with new dentin depositing along the canal walls. We found that sDPSCs are similar to their human counterparts exhibiting mesenchymal stem cell characteristics with ability to form colony forming unit-fibroblastic and odontogenic differentiation potential. sDPSCs formed pulp-dentin complex in the HA/TCP model and showed pulp regeneration capacity in the tooth fragment model. We then tested orthotopic pulp regeneration on mini-swine including the use of multi-rooted teeth. Using autologous sDPSCs carried by hydrogel and transplanted into the mini-swine root canal space, we observed regeneration of vascularized pulp-like tissue with a layer of newly deposited dentin-like (rD) tissue or osteodentin along the canal walls. In some cases, dentin bridge-like structure was observed. Immunohistochemical analysis detected the expression of nestin, dentin sialophosphoprotein, dentin matrix protein 1, and bone sialoprotein in odontoblast-like cells lining against the produced rD. We also tested the use of allogeneic sDPSCs for the same procedures. Similar findings were observed in allogeneic transplantation. This study is the first to show an establishment of mini-swine as a suitable large animal model utilizing multi-rooted teeth for further cell-based pulp regeneration studies.

Indexed as

Cell DifferentiationModels, AnimalRegenerationAnimalsDental PulpDentinFemaleHumansMiceMice, Inbred NODMice, SCIDStem CellsSwineSwine, MiniatureTissue EngineeringTissue Scaffoldsallogeneicautologousdental pulp stem cellsectopic modelmini-swineorthotopic modelpulp regenerationtooth fragment model

Identifiers

PMID29298406
PMCPMC5816511
OpenAlexW2782447519

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.