ArticleeLife2018
Multi-neuron intracellular recording in vivo via interacting autopatching robots.
Article in eLife, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- FACED 2.0 enables large-scale voltage and calcium imaging in vivo.Nature methods · 2026Article
- Robotic Fast Patch Clamp in Brain Slices Based on Stepwise Micropipette Navigation and Gigaseal Formation Control.Sensors (Basel, Switzerland) · 2025Article
- Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections.eLife · 2024Article
- Patch-walking: Coordinated multi-pipette patch clamp for efficiently finding synaptic connections.bioRxiv : the preprint server for biology · 2024Article
- High-throughput genetic manipulation of multicellular organisms using a machine-vision guided embryonic microinjection robot.Genetics · 2024Article
- Intracellular neural control of an active feeding structure in Aplysia using a carbon fiber electrode array.Journal of neuroscience methods · 2024Article
- Immediate reuse of patch-clamp pipettes after ultrasonic cleaning.Scientific reports · 2024Article
- Modeling the diverse effects of divisive normalization on noise correlations.PLoS computational biology · 2023Article
- Neuron Contact Detection Based on Pipette Precise Positioning for Robotic Brain-Slice Patch Clamps.Sensors (Basel, Switzerland) · 2023Article
- Architectural organization of ∼1,500-neuron modular minicolumnar disinhibitory circuits in healthy and Alzheimer's cortices.Cell reports · 2023Article
- Translational opportunities and challenges of invasive electrodes for neural interfaces.Nature biomedical engineering · 2023Review
- Bringing Anatomical Information into Neuronal Network Models.Advances in experimental medicine and biology · 2022Article
- Automated microscope-independent fluorescence-guided micropipette.Biomedical optics express · 2021Article
- Inferring thalamocortical monosynaptic connectivity in vivo.Journal of neurophysiology · 2021Article
- In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives.Sensors (Basel, Switzerland) · 2021Review
- Automatic deep learning-driven label-free image-guided patch clamp system.Nature communications · 2021Article
- Single neuron recording: progress towards high-throughput analysis.Bioelectronics in medicine · 2020Article
- Assembly and operation of an open-source, computer numerical controlled (CNC) robot for performing cranial microsurgical procedures.Nature protocols · 2020Article
- Article
- Robotic platform for microinjection into single cells in brain tissue.EMBO reports · 2019Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The activities of groups of neurons in a circuit or brain region are important for neuronal computations that contribute to behaviors and disease states. Traditional extracellular recordings have been powerful and scalable, but much less is known about the intracellular processes that lead to spiking activity. We present a robotic system, the multipatcher, capable of automatically obtaining blind whole-cell patch clamp recordings from multiple neurons simultaneously. The multipatcher significantly extends automated patch clamping, or 'autopatching', to guide four interacting electrodes in a coordinated fashion, avoiding mechanical coupling in the brain. We demonstrate its performance in the cortex of anesthetized and awake mice. A multipatcher with four electrodes took an average of 10 min to obtain dual or triple recordings in 29% of trials in anesthetized mice, and in 18% of the trials in awake mice, thus illustrating practical yield and throughput to obtain multiple, simultaneous whole-cell recordings in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.