ArticleBMC plant biology2017
Advantages of Amplifluor-like SNP markers over KASP in plant genotyping.
Article in BMC plant biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- EasyKASP: a simple and fast tool for KASP primer design.BMC bioinformatics · 2025Article
- An Assay for Variable Fragment Length Allele-Specific Genotyping.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Development and Validation of Kompetitive Allele-Specific Polymerase Chain Reaction Markers for Seed Protein Content in Soybean.Plants (Basel, Switzerland) · 2024Article
- Application of bulk segregant RNA-Seq (BSR-Seq) and allele-specific primers to study soybean powdery mildew resistance.BMC plant biology · 2024Article
- Evaluation of genetic diversity among olive trees (Frontiers in plant science · 2024Article
- Identification of qBK2.1, a novel QTL controlling rice resistance against Fusarium fujikuroi.Botanical studies · 2023Article
- Chile Pepper (Frontiers in plant science · 2022Review
- Expression of Specific Alleles of Zinc-Finger Transcription Factors,International journal of molecular sciences · 2021Article
- Modified "Allele-Specific qPCR" Method for SNP Genotyping Based on FRET.Frontiers in plant science · 2021Article
- Quantitative Trait Loci Mapping and Development of KASP Marker Smut Screening Assay Using High-Density Genetic Map and Bulked Segregant RNA Sequencing in Sugarcane (Frontiers in plant science · 2021Article
- Article
- Identification, gene expression and genetic polymorphism of zinc finger A20/AN1 stress-associated genes, HvSAP, in salt stressed barley from Kazakhstan.BMC plant biology · 2020Article
- Development and validation of high-throughput and low-cost STARP assays for genes underpinning economically important traits in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Appraisal of wheat genomics for gene discovery and breeding applications: a special emphasis on advances in Asia.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- Bulked segregant RNA-sequencing (BSR-seq) identified a novel rare allele of eIF4E effective against multiple isolates of BaYMV/BaMMV.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- From markers to genome-based breeding in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Review
- Intracellular Vesicle Trafficking Genes,Frontiers in genetics · 2019Article
- Article
- The General Transcription RepressorFrontiers in genetics · 2019Article
- Genes Encoding Transcription Factors TaDREB5 and TaNFYC-A7 Are Differentially Expressed in Leaves of Bread Wheat in Response to Drought, Dehydration and ABA.Frontiers in plant science · 2018Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundKASP (KBioscience Competitive Allele Specific PCR) and Amplifluor (Amplification with fluorescence) SNP markers are two prominent technologies based upon a shared identical Allele-specific PCR platform.
methodsAmplifluor-like SNP and KASP analysis was carried out using published and own design of Universal probes (UPs) and Gene-specific primers (GSPs).
resultsAdvantages of the Amplifluor-like system over KASP include the significantly lower costs and much greater flexibility in the adjustment and development of 'self-designed' dual fluorescently-labelled UPs and regular GSPs. The presented results include optimisation of 'tail' length in UPs and GSPs, protocol adjustment, and the use of various fluorophores in different qPCR instruments. The compatibility of the KASP Master-mix in both original and Amplifluor-like systems has been demonstrated in the presented results, proving their similar principles. Results of SNP scoring with rare alleles in addition to more frequently occurring alleles are shown.
conclusionsThe Amplifluor-like system produces SNP genotyping results with a level of sensitivity and accuracy comparable to KASP but at a significantly cheaper cost and with much greater flexibility for UPs with self-designed GSPs.
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