ArticleBiomedical optics express2017
Improved contrast in inverted selective plane illumination microscopy of thick tissues using confocal detection and structured illumination.
Article in Biomedical optics express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Confocal Airy beam oblique light-sheet tomography for brain-wide cell type distribution and morphology.Nature methods · 2025Article
- Self-Supervised Joint Learning for pCLE Image Denoising.Sensors (Basel, Switzerland) · 2024Article
- High contrast, isotropic, and uniform 3D-imaging of centimeter-scale scattering samples using structured illumination light-sheet microscopy with axial sweeping.Biomedical optics express · 2022Article
- Assessment of photoacoustic tomography contrast for breast tissue imaging using 3D correlative virtual histology.Scientific reports · 2022Article
- μSPIM Toolset: A software platform for selective plane illumination microscopy.Journal of neuroscience methods · 2021Article
- Emerging and Established Histological Techniques for the Analysis of Thrombosis in COVID-19 Lungs.Frontiers in cardiovascular medicine · 2021Review
- Tissue dynamics spectroscopic imaging: functional imaging of heterogeneous cancer tissue.Journal of biomedical optics · 2020Article
- Spatially modulated illumination allows for light sheet fluorescence microscopy with an incoherent source and compressive sensing.Biomedical optics express · 2019Article
- Line scanning, fiber bundle fluorescence HiLo endomicroscopy with confocal slit detection.Journal of biomedical optics · 2019Article
- Enhanced resolution 3D digital cytology and pathology with dual-view inverted selective plane illumination microscopy.Biomedical optics express · 2019Article
- Imaging neural events in zebrafish larvae with linear structured illumination light sheet fluorescence microscopy.Neurophotonics · 2019Article
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Authors and funding
3 authors.
Funding
Abstract
Inverted selective plane illumination microscopy (iSPIM) enables fast, large field-of-view, long term imaging with compatibility with conventional sample mounting. However, the imaging quality can be deteriorated in thick tissues due to sample scattering. Three strategies have been adopted in this paper to optimize the imaging performance of iSPIM on thick tissue imaging: electronic confocal slit detection (eCSD), structured illumination (SI) and the two combined. We compared the image contrast when using SPIM, confocal SPIM (using eCSD alone), SI SPIM (using SI alone) or confocal-SI SPIM (combining both methods) on images of gelatin phantom and highly-scattering fluorescently-stained human tissue. We demonstrate that all the three methods showed remarkable contrast enhancement on both samples compared to iSPIM alone, and SI SPIM and the combined confocal-SI mode outperformed confocal SPIM in contrast enhancement. Moreover, the use of SI at high pattern frequencies outperformed confocal SPIM in terms of optical sectioning capability. However, image signal-to-noise ratio (SNR) was decreased at high pattern frequencies when imaging scattering samples with SI SPIM. By combining eCSD with SI to reduce background signal and noise, the superior optical sectioning performance of SI could be achieved while also maintaining high image SNR.
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