ArticleBiomedical optics express2017
Single shot, three-dimensional fluorescence microscopy with a spatially rotating point spread function.
Article in Biomedical optics express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- PS$^{2}$2 F: Polarized Spiral Point Spread Function for Single-Shot 3D Sensing.IEEE transactions on pattern analysis and machine intelligence · 2025Article
- Simultaneous spectral differentiation of multiple fluorophores in super-resolution imaging using a glass phase plate.Optics express · 2023Article
- Monocular depth sensing using metalens.Nanophotonics (Berlin, Germany) · 2023Article
- Imaging and positioning through scattering media with double-helix point spread function engineering.Journal of biomedical optics · 2023Article
- Three-dimensional tracking using a single-spot rotating point spread function created by a multiring spiral phase plate.Journal of biomedical optics · 2022Article
- Compact light field photography towards versatile three-dimensional vision.Nature communications · 2022Article
- Reconfigurable Metalens with Phase-Change Switching between Beam Acceleration and Rotation for 3D Depth Imaging.Micromachines · 2022Article
- Three-Dimensional Incoherent Imaging Using Spiral Rotating Point Spread Functions Created by Double-Helix Beams [Invited].Nanoscale research letters · 2022Article
- High speed two-photon laser scanning stereomicroscopy for three-dimension tracking multiple particles simultaneously in three-dimension.Frontiers in photonics · 2022Article
- Hybrid multifocal structured illumination microscopy with enhanced lateral resolution and axial localization capability.Biomedical optics express · 2020Article
- Full-color optically-sectioned imaging by wide-field microscopy via deep-learning.Biomedical optics express · 2020Article
- Three-dimensional fluorescence imaging using the transport of intensity equation.Journal of biomedical optics · 2019Article
- Aberration correction method based on double-helix point spread function.Journal of biomedical optics · 2018Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
A wide-field fluorescence microscope with a double-helix point spread function (PSF) is constructed to obtain the specimen's three-dimensional distribution with a single snapshot. Spiral-phase-based computer-generated holograms (CGHs) are adopted to make the depth-of-field of the microscope adjustable. The impact of system aberrations on the double-helix PSF at high numerical aperture is analyzed to reveal the necessity of the aberration correction. A modified cepstrum-based reconstruction scheme is promoted in accordance with properties of the new double-helix PSF. The extended depth-of-field images and the corresponding depth maps for both a simulated sample and a tilted section slice of bovine pulmonary artery endothelial (BPAE) cells are recovered, respectively, verifying that the depth-of-field is properly extended and the depth of the specimen can be estimated at a precision of 23.4nm. This three-dimensional fluorescence microscope with a framerate-rank time resolution is suitable for studying the fast developing process of thin and sparsely distributed micron-scale cells in extended depth-of-field.
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Registered trials
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