ArticleMethods (San Diego, Calif.)2018
The cell free protein synthesis system from the model filamentous fungus Neurospora crassa.
Article in Methods (San Diego, Calif.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- RNA G-quadruplexes promote codon repeat-associated ribosomal frameshifting in human genes.Nucleic acids research · 2026Article
- A Pichia pastoris Cell-Free Protein Synthesis Platform.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Circadian clock control of ribosome composition promotes rhythmic translation and termination fidelity.Cell reports · 2025Article
- Review
- Cell-Free Protein Synthesis with Fungal Lysates for the Rapid Production of Unspecific Peroxygenases.Antioxidants (Basel, Switzerland) · 2022Article
- Development, validation, and application of the ribosome separation and reconstitution system for protein translation in vitro.RNA (New York, N.Y.) · 2021Article
- Structure of the translatingProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Codon usage and protein length-dependent feedback from translation elongation regulates translation initiation and elongation speed.Nucleic acids research · 2021Article
- Cell-free Translation: Preparation and Validation of Translation-competent Extracts fromBio-protocol · 2021Article
- Cell-Free Protein Synthesis: A Promising Option for Future Drug Development.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2020Review
- eRF1 mediates codon usage effects on mRNA translation efficiency through premature termination at rare codons.Nucleic acids research · 2019Article
- Cell-free microcompartmentalised transcription-translation for the prototyping of synthetic communication networks.Current opinion in biotechnology · 2019Review
- A User's Guide to Cell-Free Protein Synthesis.Methods and protocols · 2019Review
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Authors and funding
5 authors.
Funding
Abstract
Cell-free protein synthesis (CFPS) can be used in many applications to produce polypeptides and to analyze mechanisms of mRNA translation. Here we describe how to make and use a CPFS system from the model filamentous fungus Neurospora crassa. The extensive genetic resources available in this system provide capacities to exploit robust CFPS for understanding translational control. Included are procedures for the growth and harvesting of cells, the preparation of cell-free extracts that serve as the source of the translational machinery in the CFPS and the preparation of synthetic mRNA to program the CFPS. Methods to accomplish cell-free translation and analyze protein synthesis, and to map positions of ribosomes on mRNAs by toeprinting, are described.
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Registered trials
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