ArticleJournal of experimental botany2018
Novel computed tomography-based tools reliably quantify plant reproductive investment.
Article in Journal of experimental botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Phylogenetic constraints, conservatism, and convergence shape three-dimensional variation in flower morphology of a tropical orchid radiation.The Plant journal : for cell and molecular biology · 2026Article
- Preliminary study on the diagnosis of NK stress based on the puncture mechanical characteristics of cucumber stem.BMC plant biology · 2024Article
- The economy of pollen dispersal in flowering plants.Proceedings. Biological sciences · 2023Article
- Evidence for an evo-devo-derived hypothesis on three-dimensional flower shape modularity in a tropical orchid clade.Evolution; international journal of organic evolution · 2022Article
- Article
- Pollination syndrome accurately predicts pollination by tangle-veined flies (Nemestrinidae: Prosoeca s.s.) across multiple plant families.Plant biology (Stuttgart, Germany) · 2022Article
- X-ray microscopy enables multiscale high-resolution 3D imaging of plant cells, tissues, and organs.Plant physiology · 2022Article
- Advanced high-throughput plant phenotyping techniques for genome-wide association studies: A review.Journal of advanced research · 2022Review
- Functional-morphological analyses of the delicate snap-traps of the aquatic carnivorous waterwheel plant (Aldrovanda vesiculosa) with 2D and 3D imaging techniques.Annals of botany · 2020Article
- 3D Visualization and Volume-Based Quantification of Rice Chalkiness In Vivo by Using High Resolution Micro-CT.Rice (New York, N.Y.) · 2020Article
- Imaging flowers: a guide to current microscopy and tomography techniques to study flower development.Journal of experimental botany · 2020Review
- X-ray computed tomography in life sciences.BMC biology · 2020Review
- Combining high-throughput micro-CT-RGB phenotyping and genome-wide association study to dissect the genetic architecture of tiller growth in rice.Journal of experimental botany · 2019Article
- Modularity increases rate of floral evolution and adaptive success for functionally specialized pollination systems.Communications biology · 2019Article
- Beyond buzz-pollination - departures from an adaptive plateau lead to new pollination syndromes.The New phytologist · 2019Article
- JXB at SEB Florence 2018.Journal of experimental botany · 2018Article
- Quantitative imaging of plants: multi-scale data for better plant anatomy.Journal of experimental botany · 2018Article
- The lost art of looking at plants.Nature · 2018Article
- 3D pollination biology using micro-computed tomography and geometric morphometrics inApplications in plant sciencesArticle
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The flower is a bisexual reproductive unit where both genders compete for resources. Counting pollen and ovules in flowers is essential to understand how much is invested in each gender. Classical methods to count very numerous pollen grains and ovules are inefficient when pollen grains are tightly aggregated, and when fertilization rates of ovules are unknown. In this study we have therefore developed novel counting techniques based on computed tomography. In order to demonstrate the potential of our methods in very difficult cases, we counted pollen and ovules across inflorescences of deceptive and rewarding species of European orchids, which possess both very large numbers of pollen grains (tightly aggregated) and ovules. Pollen counts did not significantly vary across inflorescences and pollination strategies, whereas deceptive flowers had significantly more ovules than rewarding flowers. The within-inflorescence variance of pollen-to-ovule ratios in rewarding flowers was four times higher than in deceptive flowers, possibly demonstrating differences in the constraints acting on both pollination strategies. We demonstrate the inaccuracies and limitations of previously established methods, and the broad applicability of our new techniques: they allow measurement of reproductive investment without restriction on object number or aggregation, and without specimen destruction.
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