ArticlePloS one2018
Glutamate uptake is important for osmoregulation and survival in the rice pathogen Burkholderia glumae.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 27 citations in OpenAlex.
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- Outer membrane permeability of mcr-positive bacteria reveals potent synergy of colistin and macromolecular antibiotics against colistin-resistantFrontiers in microbiology · 2024Article
- Effects of exogenous glycine betaine and cycloleucine on photosynthetic capacity, amino acid composition, and hormone metabolism in Solanum melongena L.Scientific reports · 2023Article
- Proline confers acid stress tolerance to Bacillus megaterium G18.Scientific reports · 2022Article
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- Co-functioning of 2AP precursor amino acids enhances 2-acetyl-1-pyrroline under salt stress in aromatic rice (Oryza sativa L.) cultivars.Scientific reports · 2022Article
- Prediction and Inferred Evolution of Acid Tolerance Genes in the Biotechnologically ImportantFrontiers in microbiology · 2022Article
- Optimal NaCl Medium Enhances Squalene Accumulation inFrontiers in microbiology · 2022Article
- Modulation of Secondary Metabolites: A Halotolerance Strategy of Plant Growth Promoting Rhizobacteria Against Sodium Chloride Stress.Current microbiology · 2021Article
- Glutamate optimizes enzymatic activity under high hydrostatic pressure in Desulfovibrio species: effects on the ubiquitous thioredoxin system.Extremophiles : life under extreme conditions · 2021Article
- TAM mediates adaptation of carbapenem-resistant Klebsiella pneumoniae to antimicrobial stress during host colonization and infection.PLoS pathogens · 2021Article
- Membrane Depolarization and Apoptosis-Like Cell Death in an Alkaline Environment in the Rice PathogenFrontiers in microbiology · 2021Article
- Nitrogen Assimilation and Transport byInternational journal of molecular sciences · 2020Article
- Functional Gene Expression in Shark Bay Hypersaline Microbial Mats: Adaptive Responses.Frontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteria exhibit an optimal growth rate in culture media with sufficient nutrients at an optimal temperature and pH. In addition, the concentration of solutes plays a critical role in bacterial growth and survival. Glutamate is known to be a major anionic solute involved in osmoregulation and the bacterial cell's response to changes in solute concentration. To determine how glutamate uptake is involved in osmoregulation in the rice bacterial pathogen Burkholderia glumae BGR1, we mutated the gltI gene encoding a periplasmic substrate binding protein of a glutamate transport system to abolish glutamate uptake, and monitored the growth of the gltI null mutant in Luria-Bertani medium. We found that the gltI null mutant showed a slower growth rate than the wild-type strain and experienced hyperosmotic stress resulting in water loss from the cytoplasm in stationary phase. When the incubation time was extended, the mutant population collapsed due to the hyperosmotic stress. The gltI null mutant exhibited loss of adaptability under both hypoosmotic and hyperosmotic stresses. The growth rate of the gltI null mutant was restored to the level of wild-type growth by exogenous addition of glycine betaine to the culture medium, indicating that glycine betaine is a compatible solute in B. glumae. These results indicate that glutamate uptake from the environment plays a key role in osmoregulation in B. glumae.
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