ArticlePloS one2018
Identification of the sex-determining locus in grass puffer (Takifugu niphobles) provides evidence for sex-chromosome turnover in a subset of Takifugu species.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 58 citations in OpenAlex.
- Multiple Origins of Sex Chromosomes in Nothobranchius Killifishes.Molecular ecology · 2025Article
- Extensive Recombination Suppression and Genetic Degeneration of a Young ZW Sex Chromosome System in Halfbeak Fish.Molecular biology and evolution · 2025Article
- Sex chromosome turnover in hybridizing stickleback lineages.Evolution letters · 2024Article
- Direct male development in chromosomally ZZ zebrafish.Frontiers in cell and developmental biology · 2024Article
- Article
- Identification of a candidate sex determination gene in Culaea inconstans suggests convergent recruitment of an Amh duplicate in two lineages of stickleback.Journal of evolutionary biology · 2022Article
- Repeated translocation of a supergene underlying rapid sex chromosome turnover inProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Construction of a chromosome-level Japanese stickleback species genome using ultra-dense linkage analysis with single-cell sperm sequencing.NAR genomics and bioinformatics · 2022Article
- Something Fishy about Siamese Fighting Fish (Cells · 2022Article
- Identification of sex chromosome and sex-determining gene of southern catfish (Proceedings. Biological sciences · 2022Article
- A nonfunctional copy of the salmonid sex-determining gene (sdY) is responsible for the "apparent" XY females in Chinook salmon, Oncorhynchus tshawytscha.G3 (Bethesda, Md.) · 2022Article
- Y-specificFrontiers in genetics · 2022Article
- Heterogeneous Histories of Recombination Suppression on Stickleback Sex Chromosomes.Molecular biology and evolution · 2021Article
- Expanding the classical paradigm: what we have learnt from vertebrates about sex chromosome evolution.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- PromethION Sequencing and Assembly of the Genome of Micropoecilia picta, a Fish with a Highly Degenerated Y Chromosome.Genome biology and evolution · 2021Article
- Lessons from an unusual vertebrate sex-determining gene.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- A sex-linked SNP mutation in amhr2 is responsible for male differentiation in obscure puffer (Takifugu obscurus).Molecular biology reports · 2021Article
- How did the guppy Y chromosome evolve?PLoS genetics · 2021Article
- Genetic Dissection of a Precocious Phenotype in Male Tiger Pufferfish (Takifugu rubripes) using Genotyping by Random Amplicon Sequencing, Direct (GRAS-Di).Marine biotechnology (New York, N.Y.) · 2021Article
- Characterization of a Y-specific duplication/insertion of the anti-Mullerian hormone type II receptor gene based on a chromosome-scale genome assembly of yellow perch, Perca flavescens.Molecular ecology resources · 2020Article
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12 authors at 2 institutions in 1 country.
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Abstract
There is increasing evidence for frequent turnover in sex chromosomes in vertebrates. Yet experimental systems suitable for tracing the detailed process of turnover are rare. In theory, homologous turnover is possible if the new sex-determining locus is established on the existing sex-chromosome. However, there is no empirical evidence for such an event. The genus Takifugu includes fugu (Takifugu rubripes) and its two closely-related species whose sex is most likely determined by a SNP at the Amhr2 locus. In these species, males are heterozygous, with G and C alleles at the SNP site, while females are homozygous for the C allele. To determine if a shift in the sex-determining locus occurred in another member of this genus, we used genetic mapping to characterize the sex-chromosome systems of Takifugu niphobles. We found that the G allele of Amhr2 is absent in T. niphobles. Nevertheless, our initial mapping suggests a linkage between the phenotypic sex and the chromosome 19, which harbors the Amhr2 locus. Subsequent high-resolution analysis using a sex-reversed fish demonstrated that the sex-determining locus maps to the proximal end of chromosome 19, far from the Amhr2 locus. Thus, it is likely that homologous turnover involving these species has occurred. The data also showed that there is a male-specific reduction of recombination around the sex-determining locus. Nevertheless, no evidence for sex-chromosome differentiation was detected: the reduced recombination depended on phenotypic sex rather than genotypic sex; no X- or Y-specific maker was obtained; the YY individual was viable. Furthermore, fine-scale mapping narrowed down the new sex-determining locus to the interval corresponding to approximately 300-kb of sequence in the fugu genome. Thus, T. niphobles is determined to have a young and small sex-determining region that is suitable for studying an early phase of sex-chromosome evolution and the mechanisms underlying turnover of sex chromosome.
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