ArticlePloS one2018
Identification and evaluation of reference genes for qRT-PCR studies in Lentinula edodes.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 40 citations in OpenAlex.
- A catechol-reactive Bacillus velezensis cell-free supernatant promotes Lentinula edodes mycelial growth and elicits an iron-homeostasis-related transcriptional response.World journal of microbiology & biotechnology · 2026Article
- Transcriptomic and Metabolomic Analysis Reveals the Molecular Mechanisms of the Impact on the Fruiting Body Phenotype ofJournal of fungi (Basel, Switzerland) · 2026Article
- High-Throughput Sequencing-Guided Development and Validation of an RT-dPCR Assay for Sensitive Detection of Lentinula edodes Spherical Virus in Shiitake Mushrooms.Current microbiology · 2026Article
- Genome-wide identification of small GTPase gene family members inApplied and environmental microbiology · 2026Article
- Multi-omics analysis of polysaccharide accumulation and associated metabolic reprogramming across developmental stages ofFrontiers in microbiology · 2026Article
- Identification of stable reference genes for qRT-PCR in Stropharia rugosoannulata using mRNA-sequencing data.PloS one · 2025Article
- Transcriptome Analysis of the Growth-Promoting Effect of Large Macrofungal Sclerotium Powder onJournal of fungi (Basel, Switzerland) · 2024Article
- Homologous Delta-12 Fatty Acid Desaturase (Journal of fungi (Basel, Switzerland) · 2024Article
- Selection and validation of reference genes for RT-qPCR in ophiocordyceps sinensis under different experimental conditions.PloS one · 2024Article
- Transcriptome analysis provides insight into gamma irradiation delaying quality deterioration of postharvestFood chemistry. Molecular sciences · 2023Article
- HOG1 Mitogen-Activated Protein Kinase Pathway-Related Autophagy Induced by HJournal of fungi (Basel, Switzerland) · 2023Article
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- Enhanced Effects of Iron on Mycelial Growth, Metabolism and In Vitro Antioxidant Activity of Polysaccharides fromBioengineering (Basel, Switzerland) · 2022Article
- Selection of reference genes for quantitative PCR analysis in poultry red mite (Dermanyssus gallinae).The Journal of veterinary medical science · 2021Article
- Selection and validation of endogenous reference genes for RT-qPCR normalization in different stresses and tissues of the tiger milk mushroom,Mycoscience · 2021Article
- Article
- Identification and Validation of Reference Genes for Gene Expression Analysis in Different Development Stages ofFrontiers in microbiology · 2021Article
- Selection and validation of optimal endogenous reference genes for analysis of quantitative PCR in four tissues pathologically associated with Kidney-yang deficiency syndrome following influenza A infection.Experimental and therapeutic medicine · 2020Article
- Selection of suitable reference genes for qRT-PCR expression analysis of Codonopsis pilosula under different experimental conditions.Molecular biology reports · 2020Article
- Effect of sodium and calcium on polysaccharide production and the activities of enzymes involved in the polysaccharide synthesis of Lentinus edodes.AMB Express · 2020Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lentinula edodes (shiitake mushroom) is a common edible mushroom with a number of potential therapeutic and nutritional applications. It contains various medically important molecules, such as polysaccharides, terpenoids, sterols, and lipids, were contained in this mushroom. Quantitative real-time polymerase chain reaction (qRT-PCR) is a powerful tool to analyze the mechanisms underlying the biosynthetic pathways of these substances. qRT-PCR is used for accurate analyses of transcript levels owing to its rapidity, sensitivity, and reliability. However, its accuracy and reliability for the quantification of transcripts rely on the expression stability of the reference genes used for data normalization. To ensure the reliability of gene expression analyses using qRT-PCR in L. edodes molecular biology research, it is necessary to systematically evaluate reference genes. In the current study, ten potential reference genes were selected from L. edodes genomic data and their expression levels were measured by qRT-PCR using various samples. The expression stability of each candidate gene was analyzed by three commonly used software packages: geNorm, NormFinder, and BestKeeper. Base on the results, Rpl4 was the most stable reference gene across all experimental conditions, and Atu was the most stable gene among strains. 18S was found to be the best reference gene for different development stages, and Rpl4 was the most stably expressed gene under various nutrient conditions. The present work will contribute to qRT-PCR studies in L. edodes.
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