ArticleBiochimica et biophysica acta. Molecular cell research2018
The putative flippase Apt1 is required for intracellular membrane architecture and biosynthesis of polysaccharide and lipids in Cryptococcus neoformans.
Article in Biochimica et biophysica acta. Molecular cell research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- An experimental approach to investigate extracellular vesicle-mediated transfer of lipids between fungal cells.Microbiology spectrum · 2026Article
- Butyrolactol A enhances caspofungin efficacy via flippase inhibition in drug-resistant fungi.Cell · 2026Article
- The flippases Apt1 and Apt2 differentially influence extracellular vesicle cargo and polysaccharide secretion in Cryptococcus neoformans.Journal of proteomics · 2025Article
- Butyrolactol A potentiates caspofungin efficacy against resistant fungi via phospholipid flippase inhibition.bioRxiv : the preprint server for biology · 2025Article
- Evolutionary uniqueeLife · 2025Article
- Characterizing extracellular vesicles of human fungal pathogens.Nature microbiology · 2025Review
- Improved Broad Spectrum Antifungal Drug Synergies with Cryptomycin, a Cdc50-Inspired Antifungal Peptide.ACS infectious diseases · 2024Article
- Loss of Opi3 causes a lipid imbalance that influences the virulence traits ofFrontiers in cellular and infection microbiology · 2024Article
- Immunomodulatory Potential of Fungal Extracellular Vesicles: Insights for Therapeutic Applications.Biomolecules · 2023Review
- Characteristics and potential clinical applications of the extracellular vesicles of human pathogenic Fungi.BMC microbiology · 2023Review
- The Heat Shock Transcription Factor HsfA Plays a Role in Membrane Lipids Biosynthesis Connecting Thermotolerance and Unsaturated Fatty Acid Metabolism in Aspergillus fumigatus.Microbiology spectrum · 2023Article
- Alkaliphilic/Alkali-Tolerant Fungi: Molecular, Biochemical, and Biotechnological Aspects.Journal of fungi (Basel, Switzerland) · 2023Review
- Functional Analysis of the P-Type ATPases Apt2-4 fromJournal of fungi (Basel, Switzerland) · 2023Article
- Lipid Transport byInfection and immunity · 2022Article
- Communication is key: extracellular vesicles as mediators of infection and defence during host-microbe interactions in animals and plants.FEMS microbiology reviews · 2022Review
- P-Type ATPase Apt1 of the Fungal PathogenJournal of fungi (Basel, Switzerland) · 2021Article
- Extracellular Vesicles in the Fungi Kingdom.International journal of molecular sciences · 2021Review
- Article
- Pathogenic Delivery: The Biological Roles of Cryptococcal Extracellular Vesicles.Pathogens (Basel, Switzerland) · 2020Review
- Steryl Glycosides in Fungal Pathogenesis: An Understudied Immunomodulatory Adjuvant.Journal of fungi (Basel, Switzerland) · 2020Review
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Authors and funding
10 authors at 6 institutions in 3 countries.
Funding
Abstract
Flippases are responsible for the asymmetric distribution of phospholipids in biological membranes. In the encapsulated fungal pathogen Cryptococcus neoformans, the putative flippase Apt1 is an important regulator of polysaccharide secretion and pathogenesis in mice by unknown mechanisms. In this study, we analyzed the role of C. neoformans Apt1 in intracellular membrane architecture and synthesis of polysaccharide and lipids. Analysis of wild type (WT), apt1Δ (mutant) and apt1Δ::APT1 (complemented) strains by transmission electron microscopy revealed that deletion of APT1 resulted in the formation of irregular vacuoles. Disorganization of vacuolar membranes in apt1Δ cells was accompanied by a significant increase in the amounts of intra-vacuolar and pigment-containing vesicles. Quantitative immunogold labeling of C. neoformans cells with a monoclonal antibody raised to a major capsular component suggested impaired polysaccharide synthesis. APT1 deletion also affected synthesis of phosphatidylserine, phosphatidylethanolamine, inositolphosphoryl ceramide, glucosylceramide and ergosterylglycoside. These results reveal novel functions of Apt1 and are in agreement with the notion that this putative flippase plays an important role in the physiology of C. neoformans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.