ArticleNature biotechnology2018
Retrieval of a million high-quality, full-length microbial 16S and 18S rRNA gene sequences without primer bias.
Article in Nature biotechnology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
97 citing papers in PubMed, 3 syntheses or guidelines pooled it, 221 citations in OpenAlex.
- The microbial dark matter and "wanted list" in worldwide wastewater treatment plants.Microbiome · 2023Pooled it
- A global perspective on bacterial diversity in the terrestrial deep subsurface.Microbiology (Reading, England) · 2023Pooled it
- Pooled it
- Diversity, ecology, cell biology and evolution of the Asgard archaea.Nature reviews. Microbiology · 2026Review
- An extensive morphological and molecular characterization of the neglected class Odontostomatea (Ciliophora).Marine life science & technology · 2026Article
- Overcoming gelling agent-derived inhibitory factors enables the cultivation of fastidious nitrifiers on solid media.ISME communications · 2026Article
- Significant impact of threshold adjustments on microbiome characterization following Nanopore sequencing.ISME communications · 2026Article
- HiMBar: A High-Fidelity Metagenomic Barcoding Approach for Transkingdom Species Detection and Interaction Analysis in Aquatic Ecosystems.Molecular ecology resources · 2026Article
- Artificial sediments enable reproducible cultivation and recapitulate ecological interactions of cable bacteria.ISME communications · 2026Article
- Article
- Evaluation of long-read 16S rRNA next-generation sequencing for identification of bacterial isolates in a clinical diagnostic laboratory.Journal of clinical microbiology · 2025Article
- Characteristics of tongue images and tongue coating bacteria in patients with colorectal cancer.BMC microbiology · 2025Article
- Nanopore Environmental Analysis.JACS Au · 2025Review
- MultiTax-human: an extensive and high-resolution human-related full-length 16S rRNA reference database and taxonomy.Microbiology spectrum · 2025Article
- Effects of a Bacillus-based direct-fed microbial on digestibility, ruminal in situ nutrient disappearance, microbiome, and fermentation parameters in forage-fed beef cattle.Journal of animal science · 2025Article
- KSGP 3.1: improved taxonomic annotation of Archaea communities using LotuS2, the genome taxonomy database and RNAseq data.ISME communications · 2025Article
- Comparing the effectiveness of different DNA extraction methods in MX-80 bentonite.Environmental microbiology reports · 2024Article
- Changes in Gut Microbiota in Peruvian Cattle Genetic Nucleus by Breed and Correlations with Beef Quality.Veterinary sciences · 2024Article
- Short-read full-length 16S rRNA amplicon sequencing for characterisation of the respiratory bacteriome of captive and free-ranging African elephants (Loxodonta africana).Scientific reports · 2024Article
- Understanding the Role of Female Genital Tract Microbiome in Recurrent Implantation Failure.Journal of clinical medicine · 2024Review
37 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Small subunit ribosomal RNA (SSU rRNA) genes, 16S in bacteria and 18S in eukaryotes, have been the standard phylogenetic markers used to characterize microbial diversity and evolution for decades. However, the reference databases of full-length SSU rRNA gene sequences are skewed to well-studied ecosystems and subject to primer bias and chimerism, which results in an incomplete view of the diversity present in a sample. We combine poly(A)-tailing and reverse transcription of SSU rRNA molecules with synthetic long-read sequencing to generate high-quality, full-length SSU rRNA sequences, without primer bias, at high throughput. We apply our approach to samples from seven different ecosystems and obtain more than a million SSU rRNA sequences from all domains of life, with an estimated raw error rate of 0.17%. We observe a large proportion of novel diversity, including several deeply branching phylum-level lineages putatively related to the Asgard Archaea. Our approach will enable expansion of the SSU rRNA reference databases by orders of magnitude, and contribute to a comprehensive census of the tree of life.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.