Evidence map›Paper›PMID 29290650›Full record

ArticleWorld journal of gastroenterology2017

Structural shift of gut microbiota during chemo-preventive effects of epigallocatechin gallate on colorectal carcinogenesis in mice.

Xin Wang, Tao Ye, Wen-Jie Chen, You Lv, Zong Hao, Jun Chen, Jia-Ying Zhao, Hui-Peng Wang, Yuan-Kun Cai

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of gastroenterology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed, 2 pooled it
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 2 syntheses or guidelines pooled it, 54 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Review
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  6. Article
  7. Review
  8. Phytochemical Screening andMediators of inflammation · 2025
    Article
  9. Article
  10. Review
  11. Article
  12. Review
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  14. Review
  15. Review
  16. Article
  17. Herbal medicine and its impact on the gut microbiota in colorectal cancer.Frontiers in cellular and infection microbiology · 2023
    Review
  18. Natural Polyphenols for Treatment of Colorectal Cancer.Molecules (Basel, Switzerland) · 2022
    Review
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Xin WangDepartment of General Surgery, The 5 People's Hospital of Shanghai, Fudan University, Shanghai 200240, China.
Tao YeDepartment of General Surgery, The 5 People's Hospital of Shanghai, Fudan University, Shanghai 200240, China.
Wen-Jie ChenDepartment of General Surgery, The 5 People's Hospital of Shanghai, Fudan University, Shanghai 200240, China.
You LvDepartment of General Surgery, The 5 People's Hospital of Shanghai, Fudan University, Shanghai 200240, China.
Zong HaoDepartment of General Surgery, The 5 People's Hospital of Shanghai, Fudan University, Shanghai 200240, China.
Jun ChenDepartment of General Surgery, The 5 People's Hospital of Shanghai, Fudan University, Shanghai 200240, China.
Jia-Ying ZhaoDepartment of General Surgery, The 5 People's Hospital of Shanghai, Fudan University, Shanghai 200240, China.
Hui-Peng WangDepartment of General Surgery, The 5 People's Hospital of Shanghai, Fudan University, Shanghai 200240, China.
Yuan-Kun CaiDepartment of General Surgery, The 5 People's Hospital of Shanghai, Fudan University, Shanghai 200240, China. yuankun@medmail.com.cn.
Fudan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo investigate the effect of epigallocatechin gallate (EGCG) on structural changes of gut microbiota in colorectal carcinogenesis.

methodsAn azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colitis mouse model was established. Forty-two female FVB/N mice were randomly divided into the following three groups: group 1 (10 mice, negative control) was treated with vehicle, group 2 (16 mice, positive control) was treated with AOM plus vehicle, and group 3 (16 mice, EG) was treated with AOM plus EGCG. For aberrant crypt foci (ACF) evaluation, the colons were rapidly took out after sacrifice, rinsed with saline, opened longitudinally, laid flat on a polystyrene board, and fixed with 10% buffered formaldehyde solution before being stained with 0.2% methylene blue in saline. For tumor evaluation, the colon was macroscopically inspected and photographed, then the total number of tumors was enumerated and tumor size measured. For histological examination, the fixed tissues were paraffin-embedded and sectioned at 5 mm thickness. Microbial genomic DNA was extracted from fecal and intestinal content samples using a commercial kit. The V4 hypervariable regions of 16S rRNA were PCR-amplified with the barcoded fusion primers. Using the best hit classification option, the sequences from each sample were aligned to the RDP 16S rRNA training set to classify the taxonomic abundance in QIIME. Statistical analyses were then performed.

resultsTreatment of mice with 1% EGCG caused a significant decrease in the mean number of ACF per mouse, when compared with the model mice treated with AOM/DSS (5.38 ± 4.24

conclusionGut microbiota imbalance might be a potential mechanism for the prevention of malignant transformation by EGCG, which is significant for diagnosis, treatment, prognosis evaluation, and prevention of colorectal cancer.

Indexed as

Aberrant Crypt FociAnimalsAnticarcinogenic AgentsAzoxymethaneCarcinogenesisCarcinogensCatechinColonColorectal NeoplasmsDextran SulfateDisease Models, AnimalFemaleGastrointestinal MicrobiomeHumansMiceRectumAnticarcinogenic AgentsAzoxymethaneCarcinogensCatechinDextran Sulfateepigallocatechin gallateRNA, Ribosomal, 16SAnimal experimentChemopreventionColorectal cancerEpigallocatechin gallateGut microbiotaHigh throughput sequencing

Identifiers

PMID29290650
PMCPMC5739920
OpenAlexW2771134604

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.