ArticleAnalytica chimica acta2018
Reducing WBC background in cancer cell separation products by negative acoustic contrast particle immuno-acoustophoresis.
Article in Analytica chimica acta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 61 citations in OpenAlex.
- Recent advances in integrated biophysical and biochemical microfluidic methods for circulating tumor cells isolation and analysis.Fundamental research · 2026Review
- Development of PowerMag System II for Isolation of Circulating Tumor Cells with Improved Purity.Biomedicines · 2025Article
- Acoustic pipette and biofunctional elastomeric microparticle system for rapid picomolar-level biomolecule detection in whole blood.Science advances · 2024Article
- The integrated on-chip isolation and detection of circulating tumour cells.Sensors & diagnostics · 2024Review
- Harnessing the power of Microscale AcoustoFluidics: A perspective based on BAW cancer diagnostics.Biomicrofluidics · 2024Article
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- Magnetic Nanoparticles for Protein Separation and Purification.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Advances in novel strategies for isolation, characterization, and analysis of CTCs and ctDNA.Therapeutic advances in medical oncology · 2023Review
- Recent Advances in Microfluidic Platform for Physical and Immunological Detection and Capture of Circulating Tumor Cells.Biosensors · 2022Review
- [Recent advances in isolation and detection of circulating tumor cells with a microfluidic system].Se pu = Chinese journal of chromatography · 2022Review
- Two-Step Acoustophoresis Separation of Live Tumor Cells from Whole Blood.Analytical chemistry · 2021Article
- Negative enrichment of circulating tumor cells from unmanipulated whole blood with a 3D printed device.Scientific reports · 2021Article
- Fabrication of tunable, high-molecular-weight polymeric nanoparticlesLab on a chip · 2021Article
- Acoustic Microfluidics.Annual review of analytical chemistry (Palo Alto, Calif.) · 2020Review
- A disposable acoustofluidic chip for nano/microparticle separation using unidirectional acoustic transducers.Lab on a chip · 2020Article
- Flow induced particle separation and collection through linear array pillar microfluidics device.Biomicrofluidics · 2020Article
- Sensor-Integrated Microfluidic Approaches for Liquid Biopsies Applications in Early Detection of Cancer.Sensors (Basel, Switzerland) · 2020Review
- Sizing biological cells using a microfluidic acoustic flow cytometer.Scientific reports · 2019Article
- Advances in isolation and detection of circulating tumor cells based on microfluidics.Cancer biology & medicine · 2018Article
- The Use of Microfluidic Technology for Cancer Applications and Liquid Biopsy.Micromachines · 2018Review
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Authors and funding
5 authors at 2 institutions in 4 countries.
Funding
Abstract
Cancer cells display acoustic properties enabling acoustophoretic separation from white blood cells (WBCs) with 2-3 log suppression of the WBC background. However, a subset of WBCs has overlapping acoustic properties with cancer cells, which is why label-free acoustophoretic cancer cell isolation needs additional purification prior to analysis. This paper reports for the first time a proof of concept for continuous flow acoustophoretic negative selection of WBCs from cancer cells using negative acoustic contrast elastomeric particles (EPs) activated with CD45-antibodies that specifically bind to WBCs. The EP/WBC complexes align at the acoustic pressure anti-nodes along the channel walls while unbound cancer cells focus to the pressure node in the channel center, enabling continuous flow based depletion of WBC background in a cancer cell product. The method does not provide a single process solution for the CTC separation challenge, but provides an elegant part to a multi-step process by further reducing the WBC background in cancer cell separation products derived from an initial step of label-free acoustophoresis. We report the recorded performance of the negative selection immuno-acoustophoretic WBC depletion and cancer cell recovery. To eliminate the negative impact of the separation due to the known problems of aggregation of negative acoustic contrast particles along the sidewalls of the acoustophoresis channel and to enable continuous separation of EP/WBC complexes from cancer cells, a new acoustic actuation method has been implemented where the ultrasound frequency is scanned (1.991MHz ± 100 kHz, scan rate 200 kHz ms
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