ArticleInternational journal of pediatric otorhinolaryngology2018
Designing a tissue-engineered tracheal scaffold for preclinical evaluation.
Article in International journal of pediatric otorhinolaryngology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- Applications of Electrospinning for Tissue Engineering in Otolaryngology.The Annals of otology, rhinology, and laryngology · 2021Pooled it
- "We were already there where the narrow path …" Benign tracheal stenosis treatment: narrative review on the state of the art and future direction.Updates in surgery · 2026Review
- Organic and Synthetic Substitutes in Tracheal Reconstruction: A Scoping Review (2015-2025).Bioengineering (Basel, Switzerland) · 2025Review
- Advancements in lung regeneration: from bench to bedside.Journal of translational medicine · 2025Review
- A Contemporary Review of Trachea, Nose, and Ear Cartilage Bioengineering and Additive Manufacturing.Biomimetics (Basel, Switzerland) · 2024Review
- The Challenge of E-Spinning Sub-Millimeter Tubular Scaffolds-A Design-of-Experiments Study for Fiber Yield Improvement.Polymers · 2024Article
- Current Status and Future Outlook of Additive Manufacturing Technologies for the Reconstruction of the Trachea.Journal of functional biomaterials · 2023Review
- Development of tissue-engineered tracheal scaffold with refined mechanical properties and vascularisation for tracheal regeneration.Frontiers in bioengineering and biotechnology · 2023Article
- Biocompatible Materials in Otorhinolaryngology and Their Antibacterial Properties.International journal of molecular sciences · 2022Review
- Development and clinical translation of tubular constructs for tracheal tissue engineering: a review.European respiratory review : an official journal of the European Respiratory Society · 2021Review
- Article
- The History of Engineered Tracheal Replacements: Interpreting the Past and Guiding the Future.Tissue engineering. Part B, Reviews · 2021Article
- Motility Improvement of Biomimetic Trachea Scaffold via Hybrid 3D-Bioprinting Technology.Polymers · 2021Article
- 3D Printed Biomimetic PCL Scaffold as Framework Interspersed With Collagen for Long Segment Tracheal Replacement.Frontiers in cell and developmental biology · 2021Article
- Tissue engineering applications in otolaryngology-The state of translation.Laryngoscope investigative otolaryngology · 2020Review
- Biodegradable electrospun patch containing cell adhesion or antimicrobial compounds for trachea repair in vivo.Biomedical materials (Bristol, England) · 2020Article
- Deconstructing tissue engineered trachea: Assessing the role of synthetic scaffolds, segmental replacement and cell seeding on graft performance.Acta biomaterialia · 2020Article
- Building Scaffolds for Tubular Tissue Engineering.Frontiers in bioengineering and biotechnology · 2020Review
- Article
- Factors Influencing Poor Outcomes in Synthetic Tissue-Engineered Tracheal Replacement.Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2019Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
Abstract
objectiveRecent efforts to tissue engineer long-segment tracheal grafts have been complicated by stenosis and malacia. It has been proposed that both the mechanical characteristics and cell seeding capacity of TETG scaffolds are integral to graft performance. Our aim was to design a tracheal construct that approximates the biomechanical properties of native sheep trachea and optimizes seeding with bone marrow derived mononuclear cells prior to preclinical evaluation in an ovine model.
methodsA solution of 8% polyethylene terephthalate (PET) and 3% polyurethane (PU) was prepared at a ratio of either 8:2 or 2:8 and electrospun onto a custom stainless steel mandrel designed to match the dimensional measurements of the juvenile sheep trachea. 3D-printed porous or solid polycarbonate C-shaped rings were embedded within the scaffolds during electrospinning. The scaffolds underwent compression testing in the anterior-posterior and lateral-medial axes and the biomechanical profiles compared to that of a juvenile ovine trachea. The most biomimetic constructs then underwent vacuum seeding with ovine bone marrow derived mononuclear cells. Fluorometric DNA assay was used to quantify scaffold seeding.
resultsBoth porous and solid rings approximated the biomechanics of the native ovine trachea, but the porous rings were most biomimetic. The load-displacement curve of scaffolds fabricated from a ratio of 2:8 PET:PU most closely mimicked that of native trachea in the anterior-posterior and medial-lateral axes. Solid C-ringed scaffolds had a greater cell seeding efficiency when compared to porous ringed scaffolds (Solid: 19 × 10
conclusionA long segment tracheal graft composed of 2:8 PET:PU with solid C-rings approximates the biomechanics of the native ovine trachea and demonstrates superior cell seeding capacity of the two prototypes tested. Further preclinical studies using this graft design in vivo would inform the rational design of an optimal TETG scaffold.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.