ArticleJournal of neuroscience methods2018
A novel ex vivo method for measuring whole brain metabolism in model systems.
Article in Journal of neuroscience methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- Evolution of neurometabolic frugality in harvester ants.Proceedings. Biological sciences · 2026Article
- Optimal neuromuscular performance requires motor neuron phosphagen kinases.The Journal of physiology · 2026Article
- Methods and Guidelines for Metabolism Studies: Applications to Cancer Research.International journal of molecular sciences · 2025Review
- Measurement of oxygen consumption rate in mouse aortic tissue.Biology methods & protocols · 2025Article
- Brain energy metabolism as an underlying basis of slow and fast cognitive phenotypes in honeybees.The Journal of experimental biology · 2024Article
- Transcriptional programs mediating neuronal toxicity and altered glial-neuronal signaling in aGenome research · 2024Article
- Transcriptional programs mediating neuronal toxicity and altered glial-neuronal signaling in abioRxiv : the preprint server for biology · 2024Article
- Flight to insight: maximizing the potential ofFrontiers in molecular neuroscience · 2024Article
- Mitochondrial phosphagen kinases support the volatile power demands of motor nerve terminals.The Journal of physiology · 2023Article
- Diet-induced glial insulin resistance impairs the clearance of neuronal debris in Drosophila brain.PLoS biology · 2023Article
- Opposing effects of genetic variation in MTCH2 for obesity versus heart failure.Human molecular genetics · 2023Article
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- Precision Medicine on the Fly: Using Drosophila to Decipher Gene-Environment Interactions in Parkinson's Disease.Toxicological sciences : an official journal of the Society of Toxicology · 2021Article
- Oligomerization of Lrrk controls actin severing and α-synuclein neurotoxicity in vivo.Molecular neurodegeneration · 2021Article
- α-synuclein impairs autophagosome maturation through abnormal actin stabilization.PLoS genetics · 2021Article
- Brain Size, Metabolism, and Social Evolution.Frontiers in physiology · 2021Article
- Biocompatibility of Platinum Nanoparticles in BrainFrontiers in neuroscience · 2021Article
- Eusociality and Senescence: Neuroprotection and Physiological Resilience to Aging in Insect and Mammalian Systems.Frontiers in cell and developmental biology · 2021Review
- Comparative proteomic analysis highlights metabolic dysfunction in α-synucleinopathy.NPJ Parkinson's disease · 2020Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMany neuronal and glial diseases have been associated with changes in metabolism. Therefore, metabolic reprogramming has become an important area of research to better understand disease at the cellular level, as well as to identify targets for treatment. Model systems are ideal for interrogating metabolic questions in a tissue dependent context. However, while new tools have been developed to study metabolism in cultured cells there has been less progress towards studies in vivo and ex vivo. NEW
methodWe have developed a method using newly designed tissue restraints to adapt the Agilent XFe96 metabolic analyzer for whole brain analysis. These restraints create a chamber for Drosophila brains and other small model system tissues to reside undisrupted, while still remaining in the zone for measurements by sensor probes.
resultsThis method generates reproducible oxygen consumption and extracellular acidification rate data for Drosophila larval and adult brains. Single brains are effectively treated with inhibitors and expected metabolic readings are observed. Measuring metabolic changes, such as glycolytic rate, in transgenic larval brains demonstrates the potential for studying how genotype affects metabolism. COMPARISON WITH EXISTING METHODS AND
conclusionsCurrent methodology either utilizes whole animal chambers to measure respiration, not allowing for targeted tissue analysis, or uses technically challenging MRI technology for in vivo analysis that is not suitable for smaller model systems. This new method allows for novel metabolic investigation of intact brains and other tissues ex vivo in a quick, and simplistic way with the potential for large-scale studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.