ArticlePloS one2017
Direct exposure to mild heat promotes proliferation and neuronal differentiation of neural stem/progenitor cells in vitro.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Thermal challenge and food intake: Mutual regulatory mechanisms.Neural regeneration research · 2026Article
- What are the mechanisms that enable Radio-Frequency therapy to be effective in managing Erectile Dysfunction? A narrative review.International journal of impotence research · 2026Review
- Biocompatible Magnetopyroelectric Composite Films for Cell Stimulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Differential Temperature-Induced Responses in Immortalized Oral and Skin Keratinocytes.International journal of molecular sciences · 2025Article
- Ultrasound-Responsive Polymeric Piezoelectric Nanoparticles for Remote Activation and Neuronal Differentiation of Human Neural Stem Cells.Small science · 2025Article
- Effect of elevated temperature and hydrocortisone addition on the proliferation of fibroblasts.Histochemistry and cell biology · 2024Article
- Implication of thermal signaling in neuronal differentiation revealed by manipulation and measurement of intracellular temperature.Nature communications · 2024Article
- Protective Effects of Repetitive Transcranial Magnetic Stimulation Against Streptozotocin-Induced Alzheimer's Disease.Molecular neurobiology · 2024Article
- Neurogenic Stem Cell Niche in the Auditory Thalamus: In Vitro Evidence of Neural Stem Cells in the Rat Medial Geniculate Body.Life (Basel, Switzerland) · 2023Article
- Effect of Heat Stress on Hippocampal Neurogenesis: Insights into the Cellular and Molecular Basis of Neuroinflammation-Induced Deficits.Cellular and molecular neurobiology · 2023Review
- Prussian Blue Nanoparticle-Mediated Scalable Thermal Stimulation for In Vitro Neuronal Differentiation.Nanomaterials (Basel, Switzerland) · 2022Article
- Chronic Administration of Thymoquinone Enhances Adult Hippocampal Neurogenesis and Improves Memory in Rats Via Regulating the BDNF Signaling Pathway.Neurochemical research · 2022Article
- In vitro culture at 39 °C during hepatic maturation of human ES cells facilitates hepatocyte-like cell functions.Scientific reports · 2022Article
- Photothermal Response Induced by Nanocage-Coated Artificial Extracellular Matrix Promotes Neural Stem Cell Differentiation.Nanomaterials (Basel, Switzerland) · 2021Article
- Ginger facilitates cell migration and heat tolerance in mouse fibroblast cells.Molecular medicine reports · 2021Article
- Heat shock response enhanced by cell culture treatment in mouse embryonic stem cell-derived proliferating neural stem cells.PloS one · 2021Article
- A quantum thermometric sensing and analysis system using fluorescent nanodiamonds for the evaluation of living stem cell functions according to intracellular temperature.Nanoscale advances · 2020Article
- Salivary Immunoglobulin A Secretion and Polymeric Ig Receptor Expression in the Submandibular Glands Are Enhanced in Heat-Acclimated Rats.International journal of molecular sciences · 2020Article
- Molecular chaperones in the brain endothelial barrier: neurotoxicity or neuroprotection?FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Review
- Up-regulation of heat shock protein 27 inhibits apoptosis in lumbosacral nerve root avulsion-induced neurons.Scientific reports · 2019Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heat acclimation in rats is associated with enhanced neurogenesis in thermoregulatory centers of the hypothalamus. To elucidate the mechanisms for heat acclimation, we investigated the effects of direct mild heat exposure on the proliferation and differentiation of neural stem/progenitor cells (NSCs/NPCs). The NSCs/NPCs isolated from forebrain cortices of 14.5-day-old rat fetuses were propagated as neurospheres at either 37.0°C (control) or 38.5°C (mild heat exposure) for four days, and the effects on proliferation were investigated by MTS cell viability assay, measurement of neurosphere diameter, and counting the total number of cells. The mRNA expressions of heat shock proteins (HSPs) and brain-derived neurotrophic factor (BDNF), cAMP response element-binding (CREB) protein and Akt phosphorylation levels, and intracellular reactive oxygen species (ROS) levels were analyzed using real time PCR, Western blotting and CM-H2DCFDA assay respectively. Heat exposure under proliferation condition increased NSC/NPC viability, neurosphere diameter, and cell count. BDNF mRNA expression, CREB phosphorylation, and ROS level were also increased by heat exposure. Heat exposure increased HSP27 mRNA expression concomitant with enhanced p-Akt level. Moreover, treatment with LY294002 (a PI3K inhibitor) abolished the effects of heat exposure on NSC/NPC proliferation. Furthermore, heat exposure under differentiation conditions increased the proportion of cells positive for Tuj1 (a neuronal marker). These findings suggest that mild heat exposure increases NSC/NPC proliferation, possibly through activation of the Akt pathway, and also enhances neuronal differentiation. Direct effects of temperature on NSCs/NPCs may be one of the mechanisms involved in hypothalamic neurogenesis in heat-acclimated rats. Such heat-induced neurogenesis could also be an effective therapeutic strategy for neurodegenerative diseases.
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