ArticleJournal of visualized experiments : JoVE2017
Preparation of Meiotic Chromosome Spreads from Mouse Spermatocytes.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 27 citations in OpenAlex.
- Basal body docking failure triggers centriole clustering and elimination in mammalian spermatogenesis.EMBO reports · 2026Article
- Loss of cilia drives centriole clustering and elimination during mammalian spermatogenesis.bioRxiv : the preprint server for biology · 2025Article
- Testis-specific protein HSF5 is essential for proper chromatin organization and transcriptional reprogramming to drive pachynema progression.Zoological research · 2025Article
- An essential role of the E3 ubiquitin ligase RNF126 in ensuring meiosis I completion during spermatogenesis.Journal of advanced research · 2025Article
- L-2-hydroxyglutarate regulates centromere and heterochromatin conformation in the male germline.PLoS genetics · 2025Article
- Centrobin serves as a safeguard to guide timely centriole maturation during the cell cycle.Scientific reports · 2025Article
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- Loss of function of male-specific lethal 3 (Msl3) does not affect spermatogenesis in rodents.Developmental dynamics : an official publication of the American Association of Anatomists · 2024Article
- Characterization of BRCA2 R3052Q variant in mice supports its functional impact as a low-risk variant.Cell death & disease · 2023Article
- Genome-wide map of R-loops reveals its interplay with transcription and genome integrity during germ cell meiosis.Journal of advanced research · 2023Article
- Retinoic acid is dispensable for meiotic initiation but required for spermiogenesis in the mammalian testis.Development (Cambridge, England) · 2023Article
- Age-related elevation of O-GlcNAc causes meiotic arrest in male mice.Cell death discovery · 2023Article
- Cholesterol-Amino-Phosphate (CAP) Derived Lipid Nanoparticles for Delivery of Self-Amplifying RNA and Restoration of Spermatogenesis in Infertile Mice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Pre-meiotic deletion of PEX5 causes spermatogenesis failure and infertility in mice.Cell proliferation · 2023Article
- NAT10-mediated N4-acetylcytidine modification is required for meiosis entry and progression in male germ cells.Nucleic acids research · 2022Article
- Epigenetic, genetic and maternal effects enable stable centromere inheritance.Nature cell biology · 2022Article
- YTHDC2 is essential for pachytene progression and prevents aberrant microtubule-driven telomere clustering in male meiosis.Cell reports · 2021Article
- The CNOT4 Subunit of the CCR4-NOT Complex is Involved in mRNA Degradation, Efficient DNA Damage Repair, and XY Chromosome Crossover during Male Germ Cell Meiosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Sperm acrosome overgrowth and infertility in mice lacking chromosome 18 pachytene piRNA.PLoS genetics · 2021Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Mammalian meiosis is a dynamic developmental process that occurs in germ cells and can be studied and characterized. Using a method to spread nuclei on the surface of slides (rather than dropping them from a height), we demonstrate an optimized technique on mouse spermatocytes that was first described in 1997. This method is widely used in laboratories to study mammalian meiosis because it yields a plethora of high quality nuclei undergoing substages of prophase I. Seminiferous tubules are first placed in a hypotonic solution to swell spermatocytes. Then spermatocytes are released into a sucrose solution to create a cell suspension, and nuclei are spread onto fixative-soaked glass slides. Following immunostaining, a diversity of proteins germane to meiotic processes can be examined. For example, proteins of the synaptonemal complex, a tripartite structure that connects the chromosome axes/cores of homologs together can be easily visualized. Meiotic recombination proteins, which are involved in repair of DNA double-strand breaks by homologous recombination, can also be immunostained to evaluate progression of prophase I. Here we describe and demonstrate in detail a technique widely used to study mammalian meiosis in spermatocytes from juvenile or adult male mice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.