ArticleJournal of visualized experiments : JoVE2017
Visualization of Biofilm Formation in Candida albicans Using an Automated Microfluidic Device.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- Ellagic Acid Potentiates the Inhibitory Effects of Fluconazole AgainstAntibiotics (Basel, Switzerland) · 2024Article
- Disinfection of needleless connectors for catheters in one second using a hand-held UV device.American journal of infection control · 2024Article
- Adhesion of Candida parapsilosis to Bovine Serum Albumin under Fluid Shear.Journal of visualized experiments : JoVE · 2021Article
- Article
- Symbiotic NCR Peptide Fragments Affect the Viability, Morphology and Biofilm Formation ofInternational journal of molecular sciences · 2021Article
- The role of biofilm in the development and dissemination of ubiquitous pathogens in drinking water distribution systems: an overview of surveillance, outbreaks, and prevention.World journal of microbiology & biotechnology · 2021Review
- The Transcription Factor Stp2 Is Important forFrontiers in microbiology · 2020Article
- Application of 460 nm visible light for the elimination of Candida albicans in vitro and in vivo.Molecular medicine reports · 2018Article
- In Vitro Culturing and Screening of Candida albicans Biofilms.Current protocols in microbiology · 2018Article
- Methodologies forMicrobial cell (Graz, Austria) · 2018Review
- Synergistic Effect of Quinic Acid Derived FromFrontiers in microbiology · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Candida albicans is the most common fungal pathogen of humans, causing about 15% of hospital-acquired sepsis cases. A major virulence attribute of C. albicans is its ability to form biofilms, structured communities of cells attached to biotic and abiotic surfaces. C. albicans biofilms can form on host tissues, such as mucosal layers, and on medical devices, such as catheters, pacemakers, dentures, and joint prostheses. Biofilms pose significant clinical challenges because they are highly resistant to physical and chemical perturbations, and can act as reservoirs to seed disseminated infections. Various in vitro assays have been utilized to study C. albicans biofilm formation, such as microtiter plate assays, dry weight measurements, cell viability assays, and confocal scanning laser microscopy. All of these assays are single end-point assays, where biofilm formation is assessed at a specific time point. Here, we describe a protocol to study biofilm formation in real-time using an automated microfluidic device under laminar flow conditions. This method allows for the observation of biofilm formation as the biofilm develops over time, using customizable conditions that mimic those of the host, such as those encountered in vascular catheters. This protocol can be used to assess the biofilm defects of genetic mutants as well as the inhibitory effects of antimicrobial agents on biofilm development in real-time.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.