ArticleJournal of visualized experiments : JoVE2017
Pulling Membrane Nanotubes from Giant Unilamellar Vesicles.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- SurFlex microscopy: Measuring flexibility of surface-tethered biomolecules.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Membrane curvature association of amphipathic helix 8 drives constitutive endocytosis of GPCRs.Science advances · 2025Article
- Sterols in plant biologyCell surface (Amsterdam, Netherlands) · 2025Review
- Protein-membrane interactions: sensing and generating curvature.Trends in biochemical sciences · 2024Review
- Membrane Tubulation with a Biomembrane Force Probe.Membranes · 2023Article
- Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction.Journal of synchrotron radiation · 2023Article
- Methods to mechanically perturb and characterize GUV-based minimal cell models.Computational and structural biotechnology journal · 2023Review
- Membrane curvature sensing and stabilization by the autophagic LC3 lipidation machinery.Science advances · 2022Article
- Friction-driven membrane scission by the human ESCRT-III proteins CHMP1B and IST1.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Molecular Shape Solution for Mesoscopic Remodeling of Cellular Membranes.Annual review of biophysics · 2022Review
- Biomechanics of Neutrophil Tethers.Life (Basel, Switzerland) · 2021Review
- Lipid-Composition-Mediated Forces Can Stabilize Tubular Assemblies of I-BAR Proteins.Biophysical journal · 2021Article
- The ESCRTs - converging on mechanism.Journal of cell science · 2020Review
- Model lipid systems and their use to evaluate the phase state of biomembranes, their mechanical properties and the effect of non-conventional antibiotics: the case of daptomycin.European biophysics journal : EBJ · 2020Article
- Mechanical Tension of Biomembranes Can Be Measured by Super Resolution (STED) Microscopy of Force-Induced Nanotubes.Nano letters · 2020Article
- Dynamic and Sequential Protein Reconstitution on Negatively Curved Membranes by Giant Vesicles Fusion.Bio-protocol · 2019Article
- Combining patch-clamping and fluorescence microscopy for quantitative reconstitution of cellular membrane processes with Giant Suspended Bilayers.Scientific reports · 2019Article
- Membrane curvature induces cardiolipin sorting.Communications biology · 2019Article
- Reconstitution of immune cell interactions in free-standing membranes.Journal of cell science · 2018Article
- The role of traction in membrane curvature generation.Molecular biology of the cell · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The reshaping of the cell membrane is an integral part of many cellular phenomena, such as endocytosis, trafficking, the formation of filopodia, etc. Many different proteins associate with curved membranes because of their ability to sense or induce membrane curvature. Typically, these processes involve a multitude of proteins making them too complex to study quantitatively in the cell. We describe a protocol to reconstitute a curved membrane in vitro, mimicking a curved cellular structure, such as the endocytic neck. A giant unilamellar vesicle (GUV) is used as a model of a cell membrane, whose internal pressure and surface tension are controlled with micropipette aspiration. Applying a point pulling force on the GUV using optical tweezers creates a nanotube of high curvature connected to a flat membrane. This method has traditionally been used to measure the fundamental mechanical properties of lipid membranes, such as bending rigidity. In recent years, it has been expanded to study how proteins interact with membrane curvature and the way they affect the shape and the mechanics of membranes. A system combining micromanipulation, microinjection, optical tweezers, and confocal microscopy allows measurement of membrane curvature, membrane tension, and the surface density of proteins, concurrently. From these measurements, many important mechanical and morphological properties of the protein-membrane system can be inferred. In addition, we lay out a protocol of creating GUVs in the presence of physiological salt concentration, and a method of quantifying the surface density of proteins on the membrane from fluorescence intensities of labeled proteins and lipids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.