ArticleJournal of visualized experiments : JoVE2017
In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- Studying the mechanisms of neurodegeneration:Frontiers in cellular neuroscience · 2025Review
- Phosphorylation of Optineurin by protein kinase D regulates Parkin-dependent mitophagy.iScience · 2024Article
- Spautin-1 promotes PINK1-PRKN-dependent mitophagy and improves associative learning capability in an alzheimer disease animal model.Autophagy · 2024Article
- Combined use of multiparametric high-content-screening and in vitro circadian reporter assays in neurotoxicity evaluation.Archives of toxicology · 2024Article
- Mitochondrial-Derived Vesicles: The Good, the Bad, and the Ugly.International journal of molecular sciences · 2023Review
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- Methods to Study Mitochondria: Techniques Used to Study the Effects of Age-Related Diseases Including Alzheimer's.Current protocols · 2023Article
- Article
- Quantification of mitophagy using mKeima-mito in cultured human primary retinal pigment epithelial cells.Experimental eye research · 2022Article
- Insights Into the Role of Mitochondria in Vascular Calcification.Frontiers in cardiovascular medicine · 2022Review
- GAK and PRKCD are positive regulators of PRKN-independent mitophagy.Nature communications · 2021Article
- Hypoxic postconditioning promotes mitophagy against transient global cerebral ischemia via PINK1/Parkin-induced mitochondrial ubiquitination in adult rats.Cell death & disease · 2021Article
- Delineating the Role of Mitophagy Inducers for Alzheimer Disease Patients.Aging and disease · 2021Review
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- A Glimmer of Hope: Maintain Mitochondrial Homeostasis to Mitigate Alzheimer's Disease.Aging and disease · 2020Review
- Article
- DNA damage invokes mitophagy through a pathway involving Spata18.Nucleic acids research · 2020Article
- Sensitive Measurement of Mitophagy by Flow Cytometry Using the pH-dependent Fluorescent Reporter mt-Keima.Journal of visualized experiments : JoVE · 2018Article
Corrections and comments
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Authors and funding
14 authors at 9 institutions in 6 countries.
Funding
Abstract
Mitochondria are the powerhouses of cells and produce cellular energy in the form of ATP. Mitochondrial dysfunction contributes to biological aging and a wide variety of disorders including metabolic diseases, premature aging syndromes, and neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD). Maintenance of mitochondrial health depends on mitochondrial biogenesis and the efficient clearance of dysfunctional mitochondria through mitophagy. Experimental methods to accurately detect autophagy/mitophagy, especially in animal models, have been challenging to develop. Recent progress towards the understanding of the molecular mechanisms of mitophagy has enabled the development of novel mitophagy detection techniques. Here, we introduce several versatile techniques to monitor mitophagy in human cells, Caenorhabditis elegans (e.g., Rosella and DCT-1/ LGG-1 strains), and mice (mt-Keima). A combination of these mitophagy detection techniques, including cross-species evaluation, will improve the accuracy of mitophagy measurements and lead to a better understanding of the role of mitophagy in health and disease.
Indexed as
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.