ArticleJournal of visualized experiments : JoVE2017
Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence.
Article in Journal of visualized experiments : JoVE, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed.
- Mathematical modeling reveals cell differentiation processes and progenitor kinetics necessary for proper nephrogenesis.Frontiers in cell and developmental biology · 2025Article
- Emx2 is an essential regulator of ciliated cell development across embryonic tissues.iScience · 2024Article
- (Zebra)fishing for nephrogenesis genes.Tissue barriers · 2024Review
- Esrrγa regulates nephron and ciliary development by controlling prostaglandin synthesis.Development (Cambridge, England) · 2023Article
- Zebrafish as a Model to Study Retinoic Acid Signaling in Development and Disease.Biomedicines · 2023Review
- Modeling Podocyte Ontogeny and Podocytopathies with the Zebrafish.Journal of developmental biology · 2023Review
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- Advances in Understanding the Genetic Mechanisms of Zebrafish Renal Multiciliated Cell Development.Journal of developmental biology · 2022Review
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- Article
- Kctd15 regulates nephron segment development by repressing Tfap2a activity.Development (Cambridge, England) · 2020Article
- Ppargc1a Controls Ciliated Cell Development by Regulating Prostaglandin Biosynthesis.Cell reports · 2020Article
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- Tfap2a is a novel gatekeeper of nephron differentiation during kidney development.Development (Cambridge, England) · 2019Article
- Prostaglandin signaling regulates renal multiciliated cell specification and maturation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Iroquois transcription factor irx2a is required for multiciliated and transporter cell fate decisions during zebrafish pronephros development.Scientific reports · 2019Article
- Homeogene emx1 is required for nephron distal segment development in zebrafish.Scientific reports · 2018Article
- Article
- Scaling up to studyCancer cell & microenvironment · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
In recent years, the zebrafish embryo has emerged as a popular model to study developmental biology due to traits such as ex utero embryo development and optical transparency. In particular, the zebrafish embryo has become an important organism to study vertebrate kidney organogenesis as well as multiciliated cell (MCC) development. To visualize MCCs in the embryonic zebrafish kidney, we have developed a combined protocol of whole-mount fluorescent in situ hybridization (FISH) and whole mount immunofluorescence (IF) that enables high resolution imaging. This manuscript describes our technique for co-localizing RNA transcripts and protein as a tool to better understand the regulation of developmental programs through the expression of various lineage factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.