Evidence map›Paper›PMID 29286302›Full record

ArticleInternational journal of molecular sciences2017

MicroRNA-27b Depletion Enhances Endotrophic and Intravascular Lipid Accumulation and Induces Adipocyte Hyperplasia in Zebrafish.

Chia-Chun Hsu, Chi-Yu Lai, Chiu-Ya Lin, Kun-Yun Yeh, Guor Mour Her

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
0.7field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 33 citations in OpenAlex.

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  15. Non-coding RNA crosstalk with nuclear receptors in liver disease.Biochimica et biophysica acta. Molecular basis of disease · 2021
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Chia-Chun HsuDepartment of Radiology, Buddhist Tzu Chi General Hospital, Taichung Branch, Taichung 427, Taiwan. jiajium@tzuchi.com.tw.
Chi-Yu LaiDepartment of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 202, Taiwan. c.y.stephen.lai@gmail.com.
Chiu-Ya LinDepartment of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 202, Taiwan. 10236012@ntou.edu.tw.
Kun-Yun YehDivision of Hemato-Oncology, Department of Internal Medicine, Chang-Chung Memorial Hospital, Keelung 204, Taiwan. yehtyng@gmail.com.
Guor Mour HerDepartment of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung 202, Taiwan. gmher@mail.ntou.edu.tw.
National Taiwan Ocean University · TWBuddhist Tzu Chi General Hospital · TWKeelung Chang Gung Memorial Hospital · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

miR-27b has emerged as a regulatory hub in cholesterol and lipid metabolism, and as a potential therapeutic target for treating atherosclerosis and obesity. However, the impact of miR-27b on lipid levels in vivo remains to be determined. Zebrafish lipids are normally stored as triacylglycerols (TGs) and their main storage sites are visceral, intramuscular, and subcutaneous lipid depots, and not blood vessels and liver. In this study, we applied microRNA-sponge (miR-SP) technology and generated zebrafish expressing transgenic miR-27b-SP (C27bSPs), which disrupted endogenous miR-27b activity and induced intravascular lipid accumulation (hyperlipidemia) and the early onset of nonalcoholic fatty liver disease (NAFLD) and nonalcoholic steatohepatitis (NASH). Oil Red O staining predominantly increased in the blood vessels and livers of larvae and juvenile C27bSPs, indicating that miR-27b depletion functionally promoted lipid accumulation. C27bSPs also showed an increased weight gain with larger fat pads, resulting from adipocyte hyperplasia. Molecular analysis revealed that miR-27b depletion increased the expression of genes that are associated with lipogenesis and the endoplasmic reticulum (ER). Moreover, miR-27b-SP increased peroxisome proliferator-activated receptor γ (PPAR-γ), CCAAT enhancer binding protein-α (C/EBP-α, and sterol regulatory element binding transcription factor 1c (SREBP-1c) expression and contributed to lipogenesis and adipogenesis.

conclusionOur results suggest that miR-27b-SP acts as a lipid enhancer by directly increasing the expression of several lipogenic/adipogenic transcriptional factors, resulting in increased lipogenesis and adipogenesis. In this study, miR-27b expression improved lipid metabolism in C27bSPs, which suggests that miR-27b is an important lipogenic factor in regulating early onset of hyperlipidemia and adipogenesis in zebrafish.

Indexed as

AdipocytesAdipogenesisAnimalsAnimals, Genetically ModifiedAntagomirsBase SequenceCCAAT-Enhancer-Binding ProteinsDisease Models, AnimalEmbryo, NonmammalianGene Expression Regulation, DevelopmentalGenes, ReporterGreen Fluorescent ProteinsHyperlipidemiasHyperplasiaLarvaLipid MetabolismAntagomirsCCAAT-Enhancer-Binding Proteinsenhanced green fluorescent proteinGreen Fluorescent ProteinsLuminescent ProteinsmicroRNA-27, zebrafishMicroRNAsPPAR gammaRed Fluorescent ProteinSterol Regulatory Element Binding Protein 1adipogenesislipogenesisliver steatosismicroRNA spongenonalcoholic fatty liver disease

Identifiers

PMID29286302
PMCPMC5796043
OpenAlexW2778127790

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.