ArticleStem cell reviews and reports2018
Epigenetic Erasing and Pancreatic Differentiation of Dermal Fibroblasts into Insulin-Producing Cells are Boosted by the Use of Low-Stiffness Substrate.
Article in Stem cell reviews and reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 35 citations in OpenAlex.
- Mechanical forces orchestrate the epigenetic landscape of oral mesenchymal stem/progenitor cell fate in dental and periodontal tissues.Frontiers in cell and developmental biology · 2026Review
- Decellularized testicular bio-scaffolds driveFrontiers in bioengineering and biotechnology · 2026Article
- Focusing on Mechanoregulation Axis in Fibrosis: Sensing, Transduction and Effecting.Frontiers in molecular biosciences · 2022Review
- Soft surfaces promote astrocytic differentiation of mouse embryonic neural stem cells via dephosphorylation of MRLC in the absence of serum.Scientific reports · 2021Article
- Article
- Joining European Scientific Forces to Face Pandemics.Trends in microbiology · 2021Article
- Current Advances in 3D Tissue and Organ Reconstruction.International journal of molecular sciences · 2021Review
- Use of Virus-Mimicking Nanoparticles to Investigate Early Infection Events in Upper Airway 3D Models.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Steering cell behavior through mechanobiology in 3D: A regenerative medicine perspective.Biomaterials · 2021Review
- Generation of Trophoblast-Like Cells From Hypomethylated Porcine Adult Dermal Fibroblasts.Frontiers in veterinary science · 2021Article
- A Two-Step Protocol to Erase Human Skin Fibroblasts and Convert Them into Trophoblast-like Cells.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Mechanical regulation of histone modifications and cell plasticity.Current opinion in solid state & materials science · 2020Article
- Adding a dimension to cell fate.Animal reproduction · 2020Article
- Shaping Pancreatic β-Cell Differentiation and Functioning: The Influence of Mechanotransduction.Cells · 2020Review
- Developmentally-Inspired Biomimetic Culture Models to Produce Functional Islet-Like Cells From Pluripotent Precursors.Frontiers in bioengineering and biotechnology · 2020Review
- Genome-Wide DNA Methylation Enhances Stemness in the Mechanical Selection of Tumor-Repopulating Cells.Frontiers in bioengineering and biotechnology · 2020Article
- Pluripotent Cell Models for Gonadal Research.International journal of molecular sciences · 2019Review
- New tools for cell reprogramming and conversion: Possible applications to livestock.Animal reproduction · 2019Article
- Use of a PTFE Micro-Bioreactor to Promote 3D Cell Rearrangement and Maintain High Plasticity in Epigenetically Erased Fibroblasts.Stem cell reviews and reports · 2019Article
- Unchain My Heart: Integrins at the Basis of iPSC Cardiomyocyte Differentiation.Stem cells international · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several studies have demonstrated the possibility to revert differentiation process, reactivating hypermethylated genes and facilitating cell transition to a different lineage. Beside the epigenetic mechanisms driving cell conversion processes, growing evidences highlight the importance of mechanical forces in supporting cell plasticity and boosting differentiation. Here, we describe epigenetic erasing and conversion of dermal fibroblasts into insulin-producing cells (EpiCC), and demonstrate that the use of a low-stiffness substrate positively influences these processes. Our results show a higher expression of pluripotency genes and a significant bigger decrease of DNA methylation levels in 5-azacytidine (5-aza-CR) treated cells plated on soft matrix, compared to those cultured on plastic dishes. Furthermore, the use of low-stiffness also induces a significant increased up-regulation of ten-eleven translocation 2 (Tet2) and histone acetyltransferase 1 (Hat1) genes, and more decreased histone deacetylase enzyme1 (Hdac1) transcription levels. The soft substrate also encourages morphological changes, actin cytoskeleton re-organization, and the activation of the Hippo signaling pathway, leading to yes-associated protein (YAP) phosphorylation and its cytoplasmic translocation. Altogether, this results in increased epigenetic conversion efficiency and in EpiCC acquisition of a mono-hormonal phenotype. Our findings indicate that mechano-transduction related responsed influence cell plasticity induced by 5-aza-CR and improve fibroblast differentiation toward the pancreatic lineage.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.