ArticleUltrasound in medicine & biology2018
Investigation of Optimized Treatment Conditions for Acoustic-Transfection Technique for Intracellular Delivery of Macromolecules.
Article in Ultrasound in medicine & biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- Enhancing cancer therapyLab on a chip · 2025Article
- Genetically Encoded Trensor Circuits Report HeLa Cell Treatment with Polyplexed Plasmid DNA and Small-Molecule Transfection Modulators.ACS synthetic biology · 2024Article
- Recent Advancements in High-Frequency Ultrasound Applications from Imaging to Microbeam Stimulation.Sensors (Basel, Switzerland) · 2024Review
- Low-intensity transcranial focused ultrasound suppresses pain by modulating pain-processing brain circuits.Blood · 2024Article
- Landscape of Cellular Bioeffects Triggered by Ultrasound-Induced Sonoporation.International journal of molecular sciences · 2022Review
- Sonoporation: Past, Present, and Future.Advanced materials technologies · 2022Article
- Optimization of microbubble-based DNA vaccination with low-frequency ultrasound for enhanced cancer immunotherapy.Advanced therapeutics · 2021Article
- Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach.Applied physics letters · 2021Article
- Investigation of displacement of intracranial electrode induced by focused ultrasound stimulation.IEEE transactions on instrumentation and measurement · 2021Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Manipulation of cellular functions and structures by introduction of genetic materials inside cells has been one of the most prominent research areas in biomedicine. High-frequency ultrasound acoustic-transfection has recently been developed and confirmed by intracellular delivery of small molecules into HeLa cells at the single-cell level with high cell viability. After we proved the concept underlying the acoustic-transfection technique, treatment conditions for different human cancer cell lines have been intensively investigated to further develop acoustic-transfection as a versatile and adaptable transfection method by satisfying the requirements of high-delivery efficiency and cell membrane permeability with minimal membrane disruption. To determine optimal treatment conditions for different cell lines, we developed a quantitative intracellular delivery score based on delivery efficiency, cell membrane permeability and cell viability after 4 and 20 h of treatment. The intracellular delivery of macromolecules and the simultaneous intracellular delivery of two molecules under optimal treatment conditions were successfully achieved. We found that DNA plasmid was delivered by acoustic-transfection technique into epiblast stem cells, which expressed transient mCherry fluorescence.
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Registered trials
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