Evidence map›Paper›PMID 29283392›Full record

ArticleInternational journal of molecular sciences2017

New Method for Differentiation of Granuloviruses (Betabaculoviruses) Based on Multitemperature Single Stranded Conformational Polymorphism.

Martyna Krejmer-Rabalska, Lukasz Rabalski, Marlinda Lobo de Souza, Sean D Moore, Boguslaw Szewczyk

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Martyna Krejmer-RabalskaDepartment of Recombinant Vaccines, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, 80-807 Gdansk, Poland. martyna.krejmer@biotech.ug.edu.pl.ORCID 0000-0002-5263-6081
Lukasz RabalskiDepartment of Recombinant Vaccines, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, 80-807 Gdansk, Poland. lukasz.rabalski@biotech.ug.edu.pl.ORCID 0000-0002-5292-9263
Marlinda Lobo de SouzaEmbrapa Recursos Genéticos e Biotecnologia, Parque Estacao Biológica, 70770-900 Brasilia, Brazil. marlinda.souza@embrapa.br.
Sean D MooreCitrus Research International (CRI), P.O. Box 20285, Humewood 6013, Port Elizabeth, South Africa. seanmoore@cri.co.za.ORCID 0000-0002-4155-4187
Boguslaw SzewczykDepartment of Recombinant Vaccines, Intercollegiate Faculty of Biotechnology University of Gdansk and Medical University of Gdansk, 80-807 Gdansk, Poland. boguslaw.szewczyk@biotech.ug.edu.pl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Baculoviruses have been used as biopesticides for decades. Recently, due to the excessive use of chemical pesticides there is a need for finding new agents that may be useful in biological protection. Sometimes few isolates or species are discovered in one host. In the past few years, many new baculovirus species have been isolated from environmental samples, thoroughly characterized and thanks to next generation sequencing methods their genomes are being deposited in the GenBank database. Next generation sequencing (NGS) methodology is the most certain way of detection, but it has many disadvantages. During our studies, we have developed a method based on Polymerase chain reaction (PCR) followed by Multitemperature Single Stranded Conformational Polymorphism (MSSCP) which allows for distinguishing new granulovirus isolates in only a few hours and at low-cost. On the basis of phylogenetic analysis of betabaculoviruses, representative species have been chosen. The alignment of highly conserved genes-

Indexed as

Biological AssayGenome, ViralAnimalsBaculoviridaeBase SequenceDNA, ViralHigh-Throughput Nucleotide SequencingIntercellular Signaling Peptides and ProteinsLepidopteraPhylogenyPolymorphism, Single-Stranded ConformationalProgranulinsSequence AlignmentSequence Homology, Nucleic AcidViral ProteinsDNA, ViralIntercellular Signaling Peptides and ProteinsProgranulinsViral Proteinsbaculovirus detectionbetabaculovirusgranulingranulovirus detectionlate expression factor-9MSSCPPCR

Identifiers

PMID29283392
PMCPMC5796033

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.