ArticleJournal of assisted reproduction and genetics2018
High-quality human preimplantation embryos actively influence endometrial stromal cell migration.
Article in Journal of assisted reproduction and genetics, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed.
- Immunological Role of Intrauterine Human Chorionic Gonadotropin in Infertile Women Undergoing ICSI.Journal of obstetrics and gynaecology of India · 2026Article
- Blastocyst-Secreted miR-519d-3p Modulated the Cell Viability, Apoptosis and Migration of Human Endometrial Stromal Cells by Targeting HIF1α.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- The Role of the Endometrium in Implantation: A Modern View.International journal of molecular sciences · 2024Review
- Human embryo implantation.Development (Cambridge, England) · 2023Review
- Comparison of frozen-thaw blastocyst transfer strategies in women aged 35-40 years: a retrospective study.Frontiers in endocrinology · 2023Article
- Expression and Regulation of a Novel Decidual Cells-Derived Estrogen Target during Decidualization.International journal of molecular sciences · 2022Article
- Maternal selection of human embryos in early gestation: Insights from recurrent miscarriage.Seminars in cell & developmental biology · 2022Review
- Endocrine disrupting chemicals interfere with decidualization of human primary endometrial stromal cellsFrontiers in endocrinology · 2022Article
- Are testicular sperms superior to ejaculated sperms in couples with previous ART failure due to high rate of fragmented embryos? A retrospective cohort study.Frontiers in surgery · 2022Article
- Organoids to model the endometrium: implantation and beyond.Reproduction & fertility · 2021Review
- Embryo biosensing by uterine natural killer cells determines endometrial fate decisions at implantation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- The Role of Decidual Subpopulations in Implantation, Menstruation and Miscarriage.Frontiers in reproductive health · 2021Review
- Exometabolomic Analysis of Decidualizing Human Endometrial Stromal and Perivascular Cells.Frontiers in cell and developmental biology · 2021Article
- Tracking placental development in health and disease.Nature reviews. Endocrinology · 2020Review
- High-quality human preimplantation embryos stimulate endometrial stromal cell migration via secretion of microRNA hsa-miR-320a.Human reproduction (Oxford, England) · 2020Article
- Inside the Endometrial Cell Signaling Subway: Mind the Gap(s).International journal of molecular sciences · 2018Review
- Incidence, Clinical Risk Factors, and Pregnancy Outcomes of Trophectoderm- and Inner Cell Mass-Poor-Quality Blastocysts in Single Blastocyst Transfer Cycles: A Retrospective Cohort Study.Reproductive medicine and biologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe purpose of this paper is to study whether human preimplantation embryos regulate endometrial stromal cell (hESC) migration.
methodsPrimary hESCs were isolated from fertile patients undergoing hysterectomy for benign conditions (uterine scar niche n = 3, dysmenorrhea n = 2; no hormonal treatment). Migration and proliferation assays were performed by culturing decidualized or non-decidualized hESCs in the presence of embryo conditioned medium (ECM) from high-quality embryos (fragmentation ≤ 20%) or from low-quality embryos (fragmentation > 20%) or in non-conditioned medium from the same dishes (control). ECM samples from 425 individually cultured human embryos were used in this study.
resultsECM from high-quality embryos, i.e., with a low percentage of fragmentation, actively stimulated decidualized hESC migration (p < 0.001). This effect was consistent throughout embryonic development from cleavage stage embryos with 2-7 cells (high quality vs. control; p = 0.036), 8-18 cells (high quality vs. control; p < 0.001) to morulae (high quality vs. control; p = 0.003). Additionally, linear regression analysis showed that hESC migration was influenced by embryo quality (fragmentation, β - 0.299; p = 0.025) and not developmental stage (cell number, β 0.177; p = 0.176) or maternal age (β - 0.036; p = 0.78). Opposite to decidualized hESCs, the migration response of non-decidualized hESCs was inhibited by ECM from high-quality embryos (p = 0.019). ECM from low-quality embryos, i.e., with a high percentage of fragmentation, did not cause an altered migration response in decidualized hESCs (p = 0.860) or non-decidualized hESCs (p = 0.986). Furthermore, ECM of both high- and low-quality human embryos did not influence the number of proliferating cells (p = 0.375) and the cell cycle time (p = 0.297) of non-decidualized or decidualized hESCs.
conclusionThis study reveals a mechanism by which high-quality human preimplantation embryos actively interact with the endometrium to increase their chances of successful implantation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.