ArticleParasitology research2018
Triplex real-time PCR for detection of Crithidia mellificae and Lotmaria passim in honey bees.
Article in Parasitology research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Diagnostic Techniques and Epidemiological Methods for Parasites in Beekeeping: Considerations and Perspectives.Pathogens (Basel, Switzerland) · 2026Review
- Somy evolution in the honey bee infecting trypanosomatid parasite Lotmaria passim.G3 (Bethesda, Md.) · 2025Article
- The trypanosomatid (Kinetoplastida: Trypanosomatidae) parasites in bees: A review on their environmental circulation, impacts and implications.Current research in insect science · 2025Review
- Winter Hive Debris Analysis Is Significant for Assessing the Health Status of Honeybee Colonies (Insects · 2024Article
- Ecological and social factors influence interspecific pathogens occurrence among bees.Scientific reports · 2024Article
- Frequent Parasitism of Apis mellifera by Trypanosomatids in Geographically Isolated Areas with Restricted Beekeeping Movements.Microbial ecology · 2023Article
- Development of a TaqMan qPCR assay for trypanosomatid multi-species detection and quantification in insects.Parasites & vectors · 2023Article
- The Epidemiological Situation of the Managed Honey Bee (Veterinary sciences · 2022Article
- Molecular Detection and Differentiation of Arthropod, Fungal, Protozoan, Bacterial and Viral Pathogens of Honeybees.Veterinary sciences · 2022Review
- Occurrence of Honey Bee (Frontiers in cellular and infection microbiology · 2022Article
- The Pathogens Spillover and Incidence Correlation in Bumblebees and Honeybees in Slovenia.Pathogens (Basel, Switzerland) · 2021Article
- Workflow ofInternational journal for parasitology. Parasites and wildlife · 2021Article
- Detection ofPathogens (Basel, Switzerland) · 2021Article
- Longitudinal analysis on parasite diversity in honeybee colonies: new taxa, high frequency of mixed infections and seasonal patterns of variation.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Currently, light microscopic examination of cell morphology cannot discriminate Crithidia mellificae and Lotmaria passim with 100% certainty. Here, a minor groove-binding (MGB) probe-based multiplex real-time PCR assay was developed for the simultaneous and quantitative detection of C. mellificae and L. passim in honey bees. A conserved Hymenoptera 18S rRNA gene was built in as an internal control that allows accurate detection of PCR inhibition and failure of DNA extraction. The newly developed assay was also applied to field samples. Of 21 honey bee colonies (446 bees) sampled from six counties in both central and eastern Massachusetts, 3 colonies (14.29%) and 8 bees (1.79%) were infected with L. passim, and 1 colony (4.76%) and 1 bee (0.22%) with C. mellificae. Our data showed a low rate of trypanosomatid infection, and L. passim was more prevalent than C. mellificae in honey bee samples in Massachusetts.
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