ArticleProceedings of the National Academy of Sciences of the United States of America2018
Silk-based multilayered angle-ply annulus fibrosus construct to recapitulate form and function of the intervertebral disc.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 76 citations in OpenAlex.
- Biomaterials for intervertebral disc regeneration: Niche reprogramming, precision therapeutics, and structural reconstruction.Bioactive materials · 2027Review
- Development of Intervertebral Disc Organoids through Directed Differentiation of Mesenchymal Stem Cells and Hierarchical 3D Printing.ACS nano · 2026Article
- Regenerative strategies for intervertebral disc degeneration.Journal of orthopaedic translation · 2025Review
- Review
- Textile Design of an Intervertebral Disc Replacement Device from Silk Yarn.Biomimetics (Basel, Switzerland) · 2023Article
- Biomechanical evaluation of a novel intervertebral disc repair technique for large box-shaped ruptures.Frontiers in bioengineering and biotechnology · 2023Article
- Review
- Mimicking Native Heart Tissue Physiology and Pathology in Silk Fibroin Constructs through a Perfusion-Based Dynamic Mechanical Stimulation Microdevice.Advanced healthcare materials · 2022Article
- Cell sources proposed for nucleus pulposus regeneration.JOR spine · 2021Review
- Decellularized Disc Hydrogels for hBMSCs tissue-specific differentiation and tissue regeneration.Bioactive materials · 2021Article
- Putting the Pieces in Place: Mobilizing Cellular Players to Improve Annulus Fibrosus Repair.Tissue engineering. Part B, Reviews · 2021Review
- Mechanical Stimulation and Diameter of Fiber Scaffolds Affect the Differentiation of Rabbit Annulus Fibrous Stem Cells.Tissue engineering and regenerative medicine · 2021Article
- Multiscale Regulation of the Intervertebral Disc: Achievements in Experimental, In Silico, and Regenerative Research.International journal of molecular sciences · 2021Review
- Regulation of differentiation of annulus fibrosus-derived stem cells using heterogeneous electrospun fibrous scaffolds.Journal of orthopaedic translation · 2021Article
- Advances and Prospects in Biomaterials for Intervertebral Disk Regeneration.Frontiers in bioengineering and biotechnology · 2021Review
- Intervertebral Disk Degeneration: The Microenvironment and Tissue Engineering Strategies.Frontiers in bioengineering and biotechnology · 2021Review
- Biomimetic angle-ply multi-lamellar scaffold for annulus fibrosus tissue engineering.Journal of materials science. Materials in medicine · 2020Article
- Bi-layered Tubular Microfiber Scaffolds as Functional Templates for Engineering Human Intestinal Smooth Muscle Tissue.Advanced functional materials · 2020Article
- In Vitro Culture of Human Corneal Endothelium on Non-Mulberry Silk Fibroin Films for Tissue Regeneration.Translational vision science & technology · 2020Article
- Biomaterials-Induced Stem Cells Specific Differentiation Into Intervertebral Disc Lineage Cells.Frontiers in bioengineering and biotechnology · 2020Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recapitulation of the form and function of complex tissue organization using appropriate biomaterials impacts success in tissue engineering endeavors. The annulus fibrosus (AF) represents a complex, multilamellar, hierarchical structure consisting of collagen, proteoglycans, and elastic fibers. To mimic the intricacy of AF anatomy, a silk protein-based multilayered, disc-like angle-ply construct was fabricated, consisting of concentric layers of lamellar sheets. Scanning electron microscopy and fluorescence image analysis revealed cross-aligned and lamellar characteristics of the construct, mimicking the native hierarchical architecture of the AF. Induction of secondary structure in the silk constructs was confirmed by infrared spectroscopy and X-ray diffraction. The constructs showed a compressive modulus of 499.18 ± 86.45 kPa. Constructs seeded with porcine AF cells and human mesenchymal stem cells (hMSCs) showed ∼2.2-fold and ∼1.7-fold increases in proliferation on day 14, respectively, compared with initial seeding. Biochemical analysis, histology, and immunohistochemistry results showed the deposition of AF-specific extracellular matrix (sulfated glycosaminoglycan and collagen type I), indicating a favorable environment for both cell types, which was further validated by the expression of AF tissue-specific genes. The constructs seeded with porcine AF cells showed ∼11-, ∼5.1-, and ∼6.7-fold increases in col I
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.