ArticleParasites & vectors2017
Acanthamoeba-mediated cytopathic effect correlates with MBP and AhLBP mRNA expression.
Article in Parasites & vectors, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- Neuroinvasive Free-Living Amoebae Pathogenesis, Neuroinflammation and Therapeutic Challenges.International journal of molecular sciences · 2026Review
- Calcium ions in tap water may increase the adhesion ability of Acanthamoeba, potentially enhancing its cytopathic effects on corneal cells.Parasite (Paris, France) · 2025Article
- Characterization of novel extracellular proteases produced by Acanthamoeba castellanii after contact with human corneal epithelial cells and their relevance to pathogenesis.Parasites & vectors · 2024Article
- Taurine, a Component of the Tear Film, Exacerbates the Pathogenic Mechanisms ofPathogens (Basel, Switzerland) · 2023Article
- Biological characteristics and pathogenicity ofFrontiers in microbiology · 2023Review
- Article
- Identification and biochemical characterisation of Acanthamoeba castellanii cysteine protease 3.Parasites & vectors · 2020Article
- Validation of reference genes for the normalization of RT-qPCR gene expression in Acanthamoeba spp.Scientific reports · 2020Article
- Paradigms of Protist/Bacteria Symbioses Affecting Human Health:Frontiers in microbiology · 2020Review
- Review
- Acanthamoeba species from a post-covid patient with CSF rhinorrhea; a next possible post covid menace? A case report.Indian journal of medical microbiologyArticle
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
backgroundIn recent years, the concern of Acanthamoeba keratitis has increased since the infection is often associated with contact lens use. Partial 18S rRNA genotypic identification of Acanthamoeba isolates is important to correlate with pathophysiological properties in order to evaluate the degree of virulence. This is the first report of genotypic identification for clinical isolates of Acanthamoeba from corneal scrapings of keratitis in Malaysia. This study is also the first to correlate the mRNA expression of MBP and AhLBP as virulent markers for axenic strains of Acanthamoeba.
resultsIn this study, ten clinical isolates were obtained from corneal scrapings. Rns genotype and intra-genotypic variation at the DF3 region of the isolates were identified. Results revealed that all clinical isolates belonged to the T4 genotype, with T4/6 (4 isolates), T4/2 (3 isolates), T4/16 (2 isolates) and one new genotype T4 sequence (T4/36), being determined. The axenic clinical isolates were cytopathogenic to rabbit corneal fibroblasts. MBP and AhLBP mRNA expression are directly correlated to Acanthamoeba cytopathic effect.
conclusionsAll ten Malaysian clinical isolates were identified as genotype T4 which is predominantly associated with AK. Measuring the mRNA expression of Acanthamoeba virulent markers could be useful in the understanding of the pathogenesis of Acanthamoeba keratitis.
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