ArticleBMC infectious diseases2017
ORFeome-based identification of biomarkers for serodiagnosis of Mycobacterium tuberculosis latent infection.
Article in BMC infectious diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Clinical Features of Invasive Fungal Disease in China Tertiary Hospital: A Prospective, Multicenter Study.Mycopathologia · 2025Article
- Diagnostic accuracy of the Enferplex Bovine TB antibody test using individual milk samples from cattle.PloS one · 2024Article
- Article
- emPAI-assisted strategy enhances screening and assessment of Mycobacterium tuberculosis infection serological markers.Microbial biotechnology · 2021Article
- Development and Evaluation of a Fusion Polyprotein Based on HspX and Other Antigen Sequences for the Serodiagnosis of Tuberculosis.Frontiers in immunology · 2021Article
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5 authors.
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Abstract
backgroundThe challenges posed by Mycobacterium tuberculosis infection require the gradual removal of the pool of latent tuberculosis infection (LTBI). The current cell-immune-based diagnostic tests used to identify LTBI individuals have several irreversible drawbacks. In the present study, we attempted to identify novel diagnostic antigens for LTBI.
methodsA high-throughput glutathione S-transferase (GST)-fusion technology was used to express over 409 TB proteins and sera from LTBI and healthy individuals was used to interrogate these GST-TB fusion proteins.
resultsOf 409 TB proteins, sixty-three reacted seropositive and defined the immuno-ORFeome of latent M. tuberculosis. Within the immuno-ORFeome, the rare targets were predominantly latency-associated proteins and secreted proteins, while the preferentially recognized antigens tended to be transmembrane proteins. Six of novel highly-reactive antigens had the potential to distinguish LTBI from active TB and healthy individuals. A multiple-antigen combination set was selected through analysis of various combinations. A panel of 94 archived serum samples was used to validate the diagnostic performance of the multiple-antigen combination set, which had sensitivity of 66.1% (95% CI 52.9, 77.4) and specificity of 87.5% (95% CI 70.1, 95.1).
conclusionThese results provide experimental evidence of the immunogenicity of novel TB proteins that are suitable for the development of serodiagnostic tools for LTBI.
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