ArticleBMC plant biology2017
Small RNA profiling for identification of miRNAs involved in regulation of saponins biosynthesis in Chlorophytum borivilianum.
Article in BMC plant biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Integrated miRNAome, transcriptome, and degradome analyses reveal the role of miRNA-mRNA modules in the biosynthesis of oridonin inFrontiers in plant science · 2025Article
- Changes in MicroRNAs expression mediate molecular mechanism underlying the effect of MeJA on the biosynthesis of podophyllotoxin in Linum album cells.Scientific reports · 2024Article
- Genome-wide analysis of Triticum aestivum bromodomain gene family and expression analysis under salt stress.Planta · 2024Article
- Fine-tuning plant valuable secondary metabolite biosynthesis via small RNA manipulation: strategies and potential.Planta · 2024Review
- Integrated mRNA and small RNA sequencing reveals post-transcriptional regulation of the sesquiterpene pathway in3 Biotech · 2023Article
- Increasing the production of the bioactive compounds in medicinal mushrooms: an omics perspective.Microbial cell factories · 2023Review
- An insight into microRNA biogenesis and its regulatory role in plant secondary metabolism.Plant cell reports · 2022Review
- Biosynthesis of Secondary Metabolites Based on the Regulation of MicroRNAs.BioMed research international · 2022Review
- Integrative analysis of microRNAs and mRNAs reveals the regulatory networks of triterpenoid saponin metabolism in Soapberry (Frontiers in plant science · 2022Article
- Identification of microRNAs from Medicinal PlantPlants (Basel, Switzerland) · 2021Article
- MicroRNAs Roles in Plants Secondary Metabolism.Plant signaling & behavior · 2021Review
- Article
- Identification of novel microRNAs and their targets in Chlorophytum borivilianum by small RNA and degradome sequencing.Non-coding RNA research · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
backgroundMicroRNAs act as molecular regulator of cell signaling, plant growth and development, and regulate various primary and secondary plant metabolic processes. In the present study, deep sequencing of small RNAs was carried out to identify known and novel miRNAs from pharmaceutically important plant, Chlorophytum borivilianum.
resultsTotal 442 known miRNAs and 5 novel miRNAs were identified from young leaf small RNA library. Experimental validation with stem loop RT-PCR confirmed the in silico identification. Based on transcriptome data of root and leaf of C. borivilianum, Oryza sativa, and Arabidopsis thaliana target gene prediction was done using psRNAtarget and mirRanda. BLAST2GO helped in localization of predicted targets and KEGG (Kyoto Encyclopedia for Genes and Genomes) pathway analysis concluded that miR9662, miR894, miR172, and miR166 might be involved in regulating saponin biosynthetic pathway. The correlation between miRNA and its target gene was further validated by RT-qPCR analysis.
conclusionThis study provides first elaborated glimpse of miRNA pool of C. borivilianum, which can help to understand the miRNA dependent regulation of saponin biosynthesis and to design further metabolic engineering experiment to enhance their contents in the plant.
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