ArticlePLoS neglected tropical diseases2017
Development of a Multilocus Sequence Typing (MLST) scheme for Treponema pallidum subsp. pertenue: Application to yaws in Lihir Island, Papua New Guinea.
Article in PLoS neglected tropical diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Trial of Three Rounds of Mass Azithromycin Administration for Yaws Eradication.The New England journal of medicine · 2022Trial
- Formal description ofInternational journal of systematic and evolutionary microbiology · 2026Article
- Article
- Low genetic diversity of Treponema pallidum ssp. pertenue (TPE) isolated from patients' ulcers in Namatanai District of Papua New Guinea: Local human population is infected by three TPE genotypes.PLoS neglected tropical diseases · 2024Article
- A Systematic Review on Suitability of Molecular Techniques for Diagnosis and Research into Infectious Diseases of Concern in Resource-Limited Settings.Current issues in molecular biology · 2022Review
- Evolutionary Processes in the Emergence and Recent Spread of the Syphilis Agent, Treponema pallidum.Molecular biology and evolution · 2022Article
- Treponema pallidum Macrolide Resistance and Molecular Epidemiology in Southern Africa, 2008 to 2018.Journal of clinical microbiology · 2021Article
- Strain diversity of Treponema pallidum subsp. pertenue suggests rare interspecies transmission in African nonhuman primates.Scientific reports · 2019Article
- Primary Syphilis in the Male Urethra: A Case Report.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Article
- Article
- Multiple Class I and Class II Haemophilus ducreyi Strains Cause Cutaneous Ulcers in Children on an Endemic Island.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2018Article
- Complete genome sequences of two strains of Treponema pallidum subsp. pertenue from Indonesia: Modular structure of several treponemal genes.PLoS neglected tropical diseases · 2018Article
- Re-emergence of yaws after single mass azithromycin treatment followed by targeted treatment: a longitudinal study.Lancet (London, England) · 2018Article
- Gene target selection for loop-mediated isothermal amplification for rapid discrimination of Treponema pallidum subspecies.PLoS neglected tropical diseases · 2018Article
- Article
- Molecular strain typing of the yaws pathogen, Treponema pallidum subspecies pertenue.PloS one · 2018Article
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Authors and funding
5 authors at 3 institutions in 4 countries.
Funding
Abstract
backgroundYaws is a neglected tropical disease, caused by Treponema pallidum subsp. pertenue. The disease causes chronic lesions, primarily in young children living in remote villages in tropical climates. As part of a global yaws eradication campaign initiated by the World Health Organization, we sought to develop and evaluate a molecular typing method to distinguish different strains of T. pallidum subsp. pertenue for disease control and epidemiological purposes. METHODS AND PRINCIPAL
findingsPublished genome sequences of strains of T. pallidum subsp. pertenue and pallidum were compared to identify polymorphic genetic loci among the strains. DNA from a number of existing historical Treponema isolates, as well as a subset of samples from yaws patients collected in Lihir Island, Papua New Guinea, were analyzed using these targets. From these data, three genes (tp0548, tp0136 and tp0326) were ultimately selected to give a high discriminating capability among the T. pallidum subsp. pertenue samples tested. Intragenic regions of these three target genes were then selected to enhance the discriminating capability of the typing scheme using short readily amplifiable loci. This 3-gene multilocus sequence typing (MLST) method was applied to existing historical human yaws strains, the Fribourg-Blanc simian isolate, and DNA from 194 lesion swabs from yaws patients on Lihir Island, Papua New Guinea. Among all samples tested, fourteen molecular types were identified, seven of which were found in patient samples and seven among historical isolates or DNA. Three types (JG8, TD6, and SE7) were predominant on Lihir Island.
conclusionsThis MLST approach allows molecular typing and differentiation of yaws strains. This method could be a useful tool to complement epidemiological studies in regions where T. pallidum subsp. pertenue is prevalent with the overall goals of improving our understanding of yaws transmission dynamics and helping the yaws eradication campaign to succeed.
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