ArticleJournal of experimental botany2018
Increases in activity of proteasome and papain-like cysteine protease in Arabidopsis autophagy mutants: back-up compensatory effect or cell-death promoting effect?
Article in Journal of experimental botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 75 citations in OpenAlex.
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- V-ATPases and amino acid catabolism are strongly disturbed in the roots of autophagy mutants in Arabidopsis, impacting water use efficiency and tricarboxylic acid metabolism.The Plant journal : for cell and molecular biology · 2025Article
- A tissue-specific rescue strategy reveals the local roles of autophagy in leaves and seeds for resource allocation.Plant physiology · 2024Article
- Genome-Wide Analysis ofInternational journal of molecular sciences · 2024Article
- Genome-Wide Identification, Phylogeny and Expression Analysis of Subtilisin (SBT) Gene Family under Wheat Biotic and Abiotic Stress.Plants (Basel, Switzerland) · 2023Article
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- Leaf senescence: progression, regulation, and application.Molecular horticulture · 2021Review
- Current Understanding of Leaf Senescence in Rice.International journal of molecular sciences · 2021Review
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- Reproductive Suicide: Similar Mechanisms of Aging inFrontiers in cell and developmental biology · 2021Article
- Genome-Wide Investigation ofFrontiers in genetics · 2021Article
- Heat Stress-Dependent Association of Membrane Trafficking Proteins With mRNPs Is Selective.Frontiers in plant science · 2021Article
- Reserve lipids and plant autophagy.Journal of experimental botany · 2020Review
- Review
- Autophagy and Nutrients Management in Plants.Cells · 2019Review
- Quantitative Proteome Analysis ofmSystems · 2019Article
- Modeling Protein Destiny in Developing Fruit.Plant physiology · 2019Article
- Plant proteases during developmental programmed cell death.Journal of experimental botany · 2019Review
- Plant proteases and programmed cell death.Journal of experimental botany · 2019Article
- New beginnings and new ends: methods for large-scale characterization of protein termini and their use in plant biology.Journal of experimental botany · 2019Review
Corrections and comments
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Authors and funding
14 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autophagy is essential for protein degradation, nutrient recycling, and nitrogen remobilization. Autophagy is induced during leaf ageing and in response to nitrogen starvation, and is known to play a fundamental role in nutrient recycling for remobilization and seed filling. Accordingly, ageing leaves of Arabidopsis autophagy mutants (atg) have been shown to over-accumulate proteins and peptides, possibly because of a reduced protein degradation capacity. Surprisingly, atg leaves also displayed higher protease activities. The work reported here aimed at identifying the nature of the proteases and protease activities that accumulated differentially (higher or lower) in the atg mutants. Protease identification was performed using shotgun LC-MS/MS proteome analyses and activity-based protein profiling (ABPP). The results showed that the chloroplast FTSH (FILAMENTATION TEMPERATURE SENSITIVE H) and DEG (DEGRADATION OF PERIPLASMIC PROTEINS) proteases and several extracellular serine proteases [subtilases (SBTs) and serine carboxypeptidase-like (SCPL) proteases] were less abundant in atg5 mutants. By contrast, proteasome-related proteins and cytosolic or vacuole cysteine proteases were more abundant in atg5 mutants. Rubisco degradation assays and ABPP showed that the activities of proteasome and papain-like cysteine protease were increased in atg5 mutants. Whether these proteases play a back-up role in nutrient recycling and remobilization in atg mutants or act to promote cell death is discussed in relation to their accumulation patterns in the atg5 mutant compared with the salicylic acid-depleted atg5/sid2 double-mutant, and in low nitrate compared with high nitrate conditions. Several of the proteins identified are indeed known as senescence- and stress-related proteases or as spontaneous cell-death triggering factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.