Evidence map›Paper›PMID 29280613›Full record

ArticleACS applied materials & interfaces2018

Multiphoton-Polymerized 3D Protein Assay.

Richard Wollhofen, Markus Axmann, Peter Freudenthaler, Christian Gabriel, Clemens Röhrl, Herbert Stangl, Thomas A Klar, Jaroslaw Jacak

Open access · hybridAbstract read
In one paragraph

Article in ACS applied materials & interfaces, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
3.4field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 33 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Review
  9. Article
  10. Review
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Richard WollhofenInstitute of Applied Physics, Johannes Kepler University Linz , 4040 Linz, Austria.
Markus AxmannInstitute of Medical Chemistry, Center for Pathobiochemistry and Genetics, Medical University of Vienna , 1090 Vienna, Austria.
Peter FreudenthalerUpper Austrian University of Applied Sciences , Campus Linz, 4020 Linz, Austria.
Christian GabrielLudwig Boltzmann Institute for Experimental and Clinical Traumatology , 1220 Vienna, Austria.
Clemens RöhrlInstitute of Medical Chemistry, Center for Pathobiochemistry and Genetics, Medical University of Vienna , 1090 Vienna, Austria.
Herbert StanglInstitute of Medical Chemistry, Center for Pathobiochemistry and Genetics, Medical University of Vienna , 1090 Vienna, Austria.
Thomas A KlarInstitute of Applied Physics, Johannes Kepler University Linz , 4040 Linz, Austria.
Jaroslaw JacakInstitute of Applied Physics, Johannes Kepler University Linz , 4040 Linz, Austria.ORCID http://orcid.org/0000-0002-4989-1276
Johannes Kepler University of Linz · ATMedical University of Vienna · ATLudwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA · AT

Funding

Austrian Science Fund FWF P 29110
6 · The paper itself

Abstract

Multiphoton polymerization (MPP) enables 3D fabrication of micro- and nanoscale devices with complex geometries. Using MPP, we create a 3D platform for protein assays. Elevating the protein-binding sites above the substrate surface allows an optically sectioned readout, minimizing the inevitable background signal from nonspecific protein adsorption at the substrate surface. Two fluorescence-linked immunosorbent assays are demonstrated, the first one relying on streptavidin-biotin recognition and the second one on antibody recognition of apolipoprotein A1, a major constituent of high-density lipoprotein particles. Signal-to-noise ratios exceeding 1000 were achieved. The platform has high potential for 3D multiplexed recognition assays with an increased binding surface for on-chip flow cells.

Indexed as

AdsorptionAntibodiesBiotinPolymerizationProteinsStreptavidinAntibodiesBiotinProteinsStreptavidinconfocal microscopydirect laser writingfunctional polymersHDLimmunoassaytwo photon lithography

Identifiers

PMID29280613
PMCPMC5773935
OpenAlexW2777000411

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.