ArticleAdvanced biomedical research2017
Optimal DNA Isolation Method for Detection of Nontuberculous Mycobacteria by Polymerase Chain Reaction.
Article in Advanced biomedical research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Modified cetyltrimethylammonium bromide DNA extraction forThe Pan African medical journal · 2026Article
- High incidence of TB at a psychiatric hospital.IJTLD open · 2025Article
- Toward same-day detection ofFrontiers in microbiology · 2025Article
- Non-Microbiological Mycobacterial Detection Techniques for Quality Control of Biological Products: A Comprehensive Review.Microorganisms · 2024Review
- Drug resistance profiles and related gene mutations in slow-growing non-tuberculous mycobacteria isolated in regional tuberculosis reference laboratories of Iran: a three year cross-sectional study.Pathogens and global health · 2023Article
- Tuberculosis Outbreak in an Educational Institution in Henan Province, China.Frontiers in public health · 2021Article
- Assessment of a new low-cost, PCR-based strategy for high-risk human papillomavirus DNA detection for cervical cancer prevention.BMC infectious diseases · 2019Article
- Fish tank granuloma: An emerging skin disease in Iran mimicking Cutaneous Leishmaniasis.PloS one · 2019Article
- Comment on "Genetic diversity of multidrug-resistant Mycobacterium tuberculosis strains isolated from tuberculosis patients in Iran using MIRU-VNTR technique".The Kaohsiung journal of medical sciences · 2018Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNontuberculous mycobacteria (NTM) are a group of opportunistic pathogens and these are widely dispersed in water and soil resources. Identification of mycobacteria isolates by conventional methods including biochemical tests, growth rates, colony pigmentation, and presence of acid-fast bacilli is widely used, but these methods are time-consuming, labor-intensive, and may sometimes remain inconclusive. MATERIALS AND
methodsThe DNA was extracted from NTM cultures using CTAB, Chelex, Chelex + Nonidet P-40, FTA
resultsThe CTAB method showed more positive results at 1:10-1:100,000 at which the DNA amount was substantial. With the Chelex method of DNA extraction, PCR amplification was detected at 1:10 and 1:1000 dilutions.
conclusionsAccording to the electrophoresis results, the CTAB and Chelex DNA extraction methods were more successful in comparison with the others as regard producing suitable concentrations of DNA with the minimum use of PCR inhibitor.
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