Evidence map›Paper›PMID 29279741›Full record

ArticleAsian spine journal2017

Generation of Induced Pluripotent Stem Cells and Neural Stem/Progenitor Cells from Newborns with Spina Bifida Aperta.

Yohei Bamba, Masahiro Nonaka, Natsu Sasaki, Tomoko Shofuda, Daisuke Kanematsu, Hiroshi Suemizu, Yuichiro Higuchi, Ritsuko K Pooh, Yonehiro Kanemura, Hideyuki Okano and 1 more

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Article in Asian spine journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 6 institutions in 2 countries.

Yohei BambaDepartment of Physiology, Keio University School of Medicine, Tokyo, Japan.
Masahiro NonakaDepartment of Pediatric Neurosurgery, Kansai Medical University, Hirakata, Japan.
Natsu SasakiDepartment of Pediatric Neurosurgery, National Medical Center for Children and Mothers Research Institute, Tokyo, Japan.
Tomoko ShofudaDivision of Stem Cell Research, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka, Japan.
Daisuke KanematsuDivision of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka, Japan.
Hiroshi SuemizuLaboratory Animal Research Department, Biomedical Research Laboratory, Central Institute for Experimental Animals, Kawasaki, Japan.
Yuichiro HiguchiLaboratory Animal Research Department, Biomedical Research Laboratory, Central Institute for Experimental Animals, Kawasaki, Japan.
Ritsuko K PoohCRIFM Clinical Research Institute of Fetal Medicine Pooh Maternity Clinic, Osaka, Japan.
Yonehiro KanemuraDivision of Regenerative Medicine, Institute for Clinical Research, Osaka National Hospital, National Hospital Organization, Osaka, Japan.
Hideyuki OkanoDepartment of Physiology, Keio University School of Medicine, Tokyo, Japan.
Mami YamasakiDepartment of Pediatric Neurosurgery, Takatsuki General Hospital, Takatsuki, Japan.
Osaka National Hospital · JPCentral Institute for Experimental Animals · JPKeio University · JPCenter for Children · USKansai Medical University · JPTakatsuki General Hospital · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

STUDY

designWe established induced pluripotent stem cells (iPSCs) and neural stem/progenitor cells (NSPCs) from three newborns with spina bifida aperta (SBa) using clinically practical methods. PURPOSE: We aimed to develop stem cell lines derived from newborns with SBa for future therapeutic use. OVERVIEW OF LITERATURE: SBa is a common congenital spinal cord abnormality that causes defects in neurological and urological functions. Stem cell transplantation therapies are predicted to provide beneficial effects for patients with SBa. However, the availability of appropriate cell sources is inadequate for clinical use because of their limited accessibility and expandability, as well as ethical issues.

methodsFibroblast cultures were established from small fragments of skin obtained from newborns with SBa during SBa repair surgery. The cultured cells were transfected with episomal plasmid vectors encoding reprogramming factors necessary for generating iPSCs. These cells were then differentiated into NSPCs by chemical compound treatment, and NSPCs were expanded using neurosphere technology.

resultsWe successfully generated iPSC lines from the neonatal dermal fibroblasts of three newborns with SBa. We confirmed that these lines exhibited the characteristics of human pluripotent stem cells. We successfully generated NSPCs from all SBa newborn-derived iPSCs with a combination of neural induction and neurosphere technology.

conclusionsWe successfully generated iPSCs and iPSC-NSPCs from surgical samples obtained from newborns with SBa with the goal of future clinical use in patients with SBa.

Indexed as

Induced pluripotent stem cellsMyelomeningoceleNeural stem/progenitor cellsRegenerative medicineSpina bifida

Identifiers

PMID29279741
PMCPMC5738307
OpenAlexW2775862362

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.