Evidence map›Paper›PMID 29279740›Full record

ArticleAsian spine journal2017

Detection of O-Linked-N-Acetylglucosamine Modification and Its Associated Enzymes in Human Degenerated Intervertebral Discs.

Georgios Nikolaou, Aristeidis H Zibis, Apostolos H Fyllos, Antonios Katsioulis, Sotirios Sotiriou, Anastasios Kotrotsios, Markos Sgantzos, Aikaterini Vassiou, Dimitrios L Arvanitis

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In one paragraph

Article in Asian spine journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.2field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Georgios NikolaouDepartment of Anatomy, Faculty of Medicine, University of Thessaly, Larissa, Greece.
Aristeidis H ZibisDepartment of Anatomy, Faculty of Medicine, University of Thessaly, Larissa, Greece.ORCID https://orcid.org/0000-0001-7122-4317
Apostolos H FyllosDepartment of Anatomy, Faculty of Medicine, University of Thessaly, Larissa, Greece.ORCID https://orcid.org/0000-0003-1384-2074
Antonios KatsioulisDepartment of Hygiene and Epidemiology, Faculty of Medicine, University of Thessaly, Larissa, Greece.ORCID https://orcid.org/0000-0002-9337-7223
Sotirios SotiriouDepartment of Embryology, Faculty of Medicine, University of Thessaly, Larissa, Greece.
Anastasios KotrotsiosDepartment of Anatomy, Faculty of Medicine, University of Thessaly, Larissa, Greece.
Markos SgantzosDepartment of Anatomy, Faculty of Medicine, University of Thessaly, Larissa, Greece.ORCID https://orcid.org/0000-0001-6337-4534
Aikaterini VassiouDepartment of Anatomy, Faculty of Medicine, University of Thessaly, Larissa, Greece.
Dimitrios L ArvanitisDepartment of Anatomy, Faculty of Medicine, University of Thessaly, Larissa, Greece.ORCID https://orcid.org/0000-0001-6435-7461
University of Thessaly · GR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

STUDY

designHuman herniated discs were obtained from discectomy specimens for the immunohistochemical detection of O-GlcNAc and O-GlcNAcase (OGA)/O-GlcNAc transferase (OGT). PURPOSE: This study aimed to quantify the extent of O-GlcNAcylation and its associated enzymes (OGT/OGA) in human degenerated intervertebral discs. OVERVIEW OF LITERATURE: The O-GlcNAcylation of nuclear, cytoplasmic, and mitochondrial proteins as well as the effects of such post-translational modifications are currently the focus of extensive research. O-GlcNAcylation is believed to contribute to the etiology of chronic illnesses by acting as a nutrient and stress sensor in the cellular environment. Mature intervertebral disc cells are chondrocyte-like cells, and O-GlcNAc has been shown to promote chondrocyte apoptosis

methodsFifty-six specimens were fixed for 24 hours in a 10% solution of neutral-buffered formaldehyde, dehydrated, and embedded in paraffin. Tissue slices (4-µm-thick) were used for hematoxylin-eosin staining and immunohistochemistry.

resultsWe found that O-GlcNAcylation of cytoplasmic proteins was less than that of nuclear proteins in both single cells and cell clusters. Cytoplasmic O-GlcNAcylation occurs subsequent to nuclear O-GlcNAcylation and is directly proportional to disc degeneration. OGT and O-GlcNAc expression levels were identical in all specimens examined.

conclusionsO-GlcNAc and OGA/OGT expression is shown to correlate for the first time with intervertebral disc cell degeneration. Increasing disc degeneration is associated with increasing O-GlcNAcylation in both nuclear and cytoplasmic proteins in human disc cells.

Indexed as

EnzymesImmunohistochemistryIntervertebral disc degenerationSpine

Identifiers

PMID29279740
PMCPMC5738306
OpenAlexW2778287786

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.