Evidence map›Paper›PMID 29279367›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2018

Cell-specific and region-specific transcriptomics in the multiple sclerosis model: Focus on astrocytes.

Noriko Itoh, Yuichiro Itoh, Alessia Tassoni, Emily Ren, Max Kaito, Ai Ohno, Yan Ao, Vista Farkhondeh, Hadley Johnsonbaugh, Josh Burda and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 164 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
164citing papers in PubMed, 2 pooled it
14.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

164 citing papers in PubMed, 2 syntheses or guidelines pooled it, 257 citations in OpenAlex.

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  16. Deletion of the X-chromosomal geneScience translational medicine · 2025
    Article
  17. Multiple sclerosis: molecular pathogenesis and therapeutic intervention.Signal transduction and targeted therapy · 2025
    Review
  18. Article
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  20. Article

104 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Noriko ItohDepartment of Neurology, University of California, Los Angeles, CA 90095.
Yuichiro ItohDepartment of Neurology, University of California, Los Angeles, CA 90095.
Alessia TassoniDepartment of Neurology, University of California, Los Angeles, CA 90095.
Emily RenDepartment of Neurology, University of California, Los Angeles, CA 90095.
Max KaitoDepartment of Neurology, University of California, Los Angeles, CA 90095.
Ai OhnoDepartment of Neurology, University of California, Los Angeles, CA 90095.
Yan AoDepartment of Neurobiology, University of California, Los Angeles, CA 90095.
Vista FarkhondehDepartment of Neurology, University of California, Los Angeles, CA 90095.
Hadley JohnsonbaughDepartment of Neurology, University of California, Los Angeles, CA 90095.
Josh BurdaDepartment of Neurobiology, University of California, Los Angeles, CA 90095.
Michael V SofroniewDepartment of Neurobiology, University of California, Los Angeles, CA 90095.
Rhonda R VoskuhlDepartment of Neurology, University of California, Los Angeles, CA 90095; rvoskuhl@mednet.ucla.edu.
University of California, Los Angeles · US

Funding

Injectable biomaterial depots to manipulate scar and foster axon growth after SCIR01NS084030 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SOFRONIEW, MICHAEL V · 2014 to 2023
$3.0M
Neuroprotection in MS: A Cell-Specific and Region-Specific Transcriptomics ApproachR01NS109670 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI VOSKUHL, RHONDA R · 2018 to 2022
$2.2M
Parental imprinting of the X chromosome: effects on neurodegenerationR01NS096748 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI VOSKUHL, RHONDA R · 2016 to 2020
$1.9M
NINDS NIH HHS R01 NS084030NINDS NIH HHS R01 NS096748NINDS NIH HHS R01 NS109670
6 · The paper itself

Abstract

Changes in gene expression that occur across the central nervous system (CNS) during neurological diseases do not address the heterogeneity of cell types from one CNS region to another and are complicated by alterations in cellular composition during disease. Multiple sclerosis (MS) is multifocal by definition. Here, a cell-specific and region-specific transcriptomics approach was used to determine gene expression changes in astrocytes in the most widely used MS model, experimental autoimmune encephalomyelitis (EAE). Astrocyte-specific RNAs from various neuroanatomic regions were attained using RiboTag technology. Sequencing and bioinformatics analyses showed that EAE-induced gene expression changes differed between neuroanatomic regions when comparing astrocytes from spinal cord, cerebellum, cerebral cortex, and hippocampus. The top gene pathways that were changed in astrocytes from spinal cord during chronic EAE involved decreases in expression of cholesterol synthesis genes while immune pathway gene expression in astrocytes was increased. Optic nerve from EAE and optic chiasm from MS also showed decreased cholesterol synthesis gene expression. The potential role of cholesterol synthesized by astrocytes during EAE and MS is discussed. Together, this provides proof-of-concept that a cell-specific and region-specific gene expression approach can provide potential treatment targets in distinct neuroanatomic regions during multifocal neurological diseases.

Indexed as

AnimalsAstrocytesCholesterolDown-RegulationEncephalomyelitis, Autoimmune, ExperimentalGene Expression RegulationHomeostasisMiceMice, Inbred C57BLMultiple SclerosisRNA, MessengerTranscriptomeUp-RegulationCholesterolRNA, Messengerastrocytescholesterolexperimental autoimmune encephalomyelitismultiple sclerosistranscriptomics

Identifiers

PMID29279367
PMCPMC5777065
OpenAlexW2777702274

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.