ArticleWater research2018
Portable platform for rapid in-field identification of human fecal pollution in water.
Article in Water research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Multiplexing LAMP Assays: A Methodological Review and Diagnostic Application.International journal of molecular sciences · 2024Review
- Analysis of macerated ticks using Boolean logic gating colorimetric isothermal nucleic acid assays for Lyme Borrelia and Ixodes scapularis ticks.Scientific reports · 2023Article
- An overview of molecular markers for identification of non-human fecal pollution sources.Frontiers in microbiology · 2023Review
- Review
- Charge Engineering Improves the Performance of Bst DNA Polymerase Fusions.ACS synthetic biology · 2022Article
- Programming cell-free biosensors with DNA strand displacement circuits.Nature chemical biology · 2022Article
- Preparation and Use of Cellular Reagents: A Low-resource Molecular Biology Reagent Platform.Current protocols · 2022Article
- Article
- Producing molecular biology reagents without purification.PloS one · 2021Article
- A Light-Weight Practical Framework for Feces Detection and Trait Recognition.Sensors (Basel, Switzerland) · 2020Article
- A Primer on Emerging Field-Deployable Synthetic Biology Tools for Global Water Quality Monitoring.NPJ clean water · 2020Article
- Locus-patterned sequence oriented enrichment for multi-dimensional gene analysis.Chemical science · 2019Article
- Comparison of Loop-Mediated Isothermal Amplification and Real-Time PCR Assays for Detection of Strongyloides Larvae in Different Specimen Matrices.Journal of clinical microbiology · 2019Article
- Challenges and perspectives in the application of isothermal DNA amplification methods for food and water analysis.Analytical and bioanalytical chemistry · 2019Article
- Detection of a microbial source tracking marker by isothermal helicase-dependent amplification and a nucleic acid lateral-flow strip test.Scientific reports · 2019Article
- Simultaneous Detection of Different Zika Virus Lineages via Molecular Computation in a Point-of-Care Assay.Viruses · 2018Article
- Smartphone-based pathogen diagnosis in urinary sepsis patients.EBioMedicine · 2018Article
- Direct nucleic acid analysis of mosquitoes for high fidelity species identification and detection of Wolbachia using a cellphone.PLoS neglected tropical diseases · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Human fecal contamination of water is a public health risk. However, inadequate testing solutions frustrate timely, actionable monitoring. Bacterial culture-based methods are simple but typically cannot distinguish fecal host source. PCR assays can identify host sources but require expertise and infrastructure. To bridge this gap we have developed a field-ready nucleic acid diagnostic platform and rapid sample preparation methods that enable on-site identification of human fecal contamination within 80 min of sampling. Our platform relies on loop-mediated isothermal amplification (LAMP) of human-associated Bacteroides HF183 genetic markers from crude samples. Oligonucleotide strand exchange (OSD) probes reduce false positives by sequence specifically transducing LAMP amplicons into visible fluorescence that can be photographed by unmodified smartphones. Our assay can detect as few as 17 copies/ml of human-associated HF183 targets in sewage-contaminated water without cross-reaction with canine or feline feces. It performs robustly with a variety of environmental water sources and with raw sewage. We have also developed lyophilized assays and inexpensive 3D-printed devices to minimize cost and facilitate field application.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.