ArticleJournal of the American Chemical Society2018
Substrate Sorting by a Supercharged Nanoreactor.
Article in Journal of the American Chemical Society, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 72 citations in OpenAlex.
- A molecular basis for stoichiometric enzyme encapsulation in the vitamin B2 biosynthesis compartment.Nature communications · 2026Article
- Review
- Cavity acidification limits ferritin iron biomineralization.Journal of inorganic biochemistry · 2026Article
- Pore Engineering as a General Strategy to Improve Protein-Based Enzyme Nanoreactor Performance.ACS nano · 2024Article
- Towards controlling activity of a peptide asparaginyl ligase (PAL) by lumazine synthetase compartmentalization.Faraday discussions · 2024Article
- Pore engineering as a general strategy to improve protein-based enzyme nanoreactor performance.bioRxiv : the preprint server for biology · 2024Article
- Stimulus-responsive assembly of nonviral nucleocapsids.Nature communications · 2024Article
- Macromolecular Cargo Encapsulation via In Vitro Assembly of Two-Component Protein Nanoparticles.Advanced healthcare materials · 2024Article
- Engineered Protein Nanocages for Concurrent RNA and Protein Packaging In Vivo.ACS synthetic biology · 2022Article
- Multiscale simulation-guided design of enzyme bioconjugates with enhanced catalysis.Chem catalysis · 2022Article
- Electrostatically cooperative host-in-host of metal cluster ⊂ ionic organic cages in nanopores for enhanced catalysis.Nature communications · 2022Article
- Harnessing physicochemical properties of virus capsids for designing enzyme confined nanocompartments.Current opinion in virology · 2022Review
- Polyelectrolyte Encapsulation and Confinement within Protein Cage-Inspired Nanocompartments.Pharmaceutics · 2021Review
- Simulating the directional translocation of a substrate by the AAA+ motor in the 26S proteasome.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Protein cages as building blocks for superstructures.Engineering biology · 2021Article
- Molecular exclusion limits for diffusion across a porous capsid.Nature communications · 2021Article
- Facile Fabrication of Protein-Macrocycle Frameworks.Journal of the American Chemical Society · 2021Article
- Connectability of protein cages.Nanoscale advances · 2020Review
- Enzyme encapsulation by protein cages.RSC advances · 2020Review
- Structural organization of biocatalytic systems: the next dimension of synthetic metabolism.Emerging topics in life sciences · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Compartmentalization of proteases enables spatially and temporally controlled protein degradation in cells. Here we show that an engineered lumazine synthase protein cage, which possesses a negatively supercharged lumen, can exploit electrostatic effects to sort substrates for an encapsulated protease. This proteasome-like nanoreactor preferentially cleaves positively charged polypeptides over both anionic and zwitterionic substrates, inverting the inherent substrate specificity of the guest enzyme approximately 480 fold. Our results suggest that supercharged nanochambers could provide a simple and potentially general means of conferring substrate specificity to diverse encapsulated catalysts.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.