ArticleNuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms2017
Long-wavelength macromolecular crystallography - First successful native SAD experiment close to the sulfur edge.
Article in Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed.
- Facing the phase problem.IUCrJ · 2023Article
- Article
- Application of sulfur SAD to small crystals with a large asymmetric unit and anomalous substructure.Acta crystallographica. Section D, Structural biology · 2022Article
- Article
- A practical overview of molecular replacement: Clostridioides difficile PilA1, a difficult case study.Acta crystallographica. Section D, Structural biology · 2020Article
- A shared vision for macromolecular crystallography over the next five years.Structural dynamics (Melville, N.Y.) · 2019Article
- Article
- Where is crystallography going?Acta crystallographica. Section D, Structural biology · 2018Review
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5 authors.
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Abstract
Phasing of novel macromolecular crystal structures has been challenging since the start of structural biology. Making use of anomalous diffraction of natively present elements, such as sulfur and phosphorus, for phasing has been possible for some systems, but hindered by the necessity to access longer X-ray wavelengths in order to make most use of the anomalous scattering contributions of these elements. Presented here are the results from a first successful experimental phasing study of a macromolecular crystal structure at a wavelength close to the sulfur K edge. This has been made possible by the in-vacuum setup and the long-wavelength optimised experimental setup at the I23 beamline at Diamond Light Source. In these early commissioning experiments only standard data collection and processing procedures have been applied, in particular no dedicated absorption correction has been used. Nevertheless the success of the experiment demonstrates that the capability to extract phase information can be even further improved once data collection protocols and data processing have been optimised.
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