Evidence map›Paper›PMID 29276315›Full record

ArticleActa histochemica et cytochemica2017

A Device for Ribbon Collection for Array Tomography with Scanning Electron Microscopy.

Taro Koike, Yosky Kataoka, Mitsuyo Maeda, Yuji Hasebe, Yuuki Yamaguchi, Mitsuo Suga, Akira Saito, Hisao Yamada

Open access · diamondAbstract read
In one paragraph

Article in Acta histochemica et cytochemica, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 22 citations in OpenAlex.

  1. Article
  2. Review
  3. Array tomography: trails to discovery.Methods in microscopy · 2024
    Article
  4. Article
  5. Review
  6. Volume electron microscopy.Nature reviews. Methods primers · 2022
    Article
  7. Review
  8. Article
  9. Review
  10. Article
  11. Q&A: Array tomography.BMC biology · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Taro KoikeDepartment of Anatomy and Cell Science, Kansai Medical University, Hirakata City, Osaka, Japan.
Yosky KataokaMulti-Modal Microstructure Analysis Unit, RIKEN CLST-JEOL Collaboration Center, Kobe City, Hyogo, Japan.
Mitsuyo MaedaMulti-Modal Microstructure Analysis Unit, RIKEN CLST-JEOL Collaboration Center, Kobe City, Hyogo, Japan.
Yuji HasebeMulti-Modal Microstructure Analysis Unit, RIKEN CLST-JEOL Collaboration Center, Kobe City, Hyogo, Japan.
Yuuki YamaguchiMulti-Modal Microstructure Analysis Unit, RIKEN CLST-JEOL Collaboration Center, Kobe City, Hyogo, Japan.
Mitsuo SugaMulti-Modal Microstructure Analysis Unit, RIKEN CLST-JEOL Collaboration Center, Kobe City, Hyogo, Japan.
Akira SaitoInstitute of Biomedical Science Central Research Center, Kansai Medical University, Hirakata City, Osaka, Japan.
Hisao YamadaDepartment of Anatomy and Cell Science, Kansai Medical University, Hirakata City, Osaka, Japan.
JEOL (Japan) · JPKansai Medical University · JPCLS Technology (Norway) · NO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

"Array tomography" is a method used to observe the fine structure of cells and tissues in a three-dimensional view. In this method, serial ultrathin sections in the ribbon state (ribbons) are mounted on a solid substrate and observed by scanning electron microscopy (SEM). The method may also be used in conjunction with post-embedding immunocytochemistry. However, it is difficult to mount many serial ribbons on a substrate manually. We developed an inexpensive laboratory-made device that mounts ribbons by pulling a nylon fishing line and lifting the substrate up from the water in a knife boat. Using this device, we succeeded in mounting several ribbons consisting a mean of 205.6 (SD: 37.7) serial ultrathin sections on 1.25 (SD: 0.06) × 1.25 (SD: 0.06)-cm silicon substrates. Furthermore, it was confirmed that our method is suitable for ribbons derived from water-soluble resin blocks. We were also able to stain the specimens by post-embedding immunocytochemistry. Thus, our method is useful in mounting numerus sections on a substrate for array tomography with SEM.

Indexed as

array tomographypost-embedding labelingscanning electron microscopyserial ultrathin sectionthree-dimensional observation

Identifiers

PMID29276315
PMCPMC5736830
OpenAlexW2766595670

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.